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产品资料 PD-40308ES
TUNEL细胞凋亡检测试剂盒(YSFluor 640) TUNEL细胞凋亡检测试剂盒(YSFluor 640)
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TUNEL细胞凋亡检测试剂盒(YSFluor 640)

TUNEL Apoptosis Detection Kit (YSFluor™ 640)

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荧光显微镜流式细胞仪细胞凋亡研究
产品编号40308ES 可选规格3 种 配套文档1 份 来源翌圣生物 · 官网
产品编号 40308ES20
现货 $1,188.00 促$1,250.00
数量 1
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规格与询价

勾选需要的规格可一次提交;也可以逐个询价。货号会一并带入询价单。

货号 规格 价格 状态
40308ES20 20 T $1,188.00促$1,250.00 现货 询价
40308ES50 50 T $2,928.00促$3,080.00 现货 询价
40308ES60 100 T $4,318.00促$4,800.00 现货 询价
提交询价 价格为协议价,提交后由业务确认;大包装可申请试用装。

产品概述

技术参数

品牌翌圣生物 Yeasen
货号 / 型号40308ES
规格应用范围广,可以用于检测冷冻或石蜡切片中的细胞凋亡情况,也可以检测培养的贴壁细胞或悬浮细胞的凋亡情况。
产地翌圣生物科技(上海)股份有限公司
技术平台 荧光显微镜 流式细胞仪
应用方法 细胞凋亡研究检测组织或细胞样本中的凋亡情况
检测方法 TUNEL法
生物特性参数 检测靶标凋亡细胞中断裂的基因组DNA
样本类型 冷冻切片 石蜡切片 贴壁细胞 悬浮细胞
技术参数 储存条件-20℃避光保存 运输条件冰袋运输 保质期1年
实验类型 TUNEL凋亡检测检测细胞凋亡晚期DNA断裂

常见问题

Q:血管内皮细胞,想要免疫荧光和 TUNEL 同时进行,不知道该先做哪个? TUNEL 要求立即检测?

A:先做 TUNEL,再进行免疫荧光,这个主要是怕免疫荧光对 TdT 酶产生影响。只要避光保存就可以不立即进行检测。

Q:感觉 DNA 染的有点弥散状,核定位的效果也不是很好,是染色时间太久?感觉细胞质中也像是染到了?

A:有非特异性染色,可能是因为时间太久或者是试剂浓度太高导致的。建议降低染色液浓度或减少染色时间。

文档与文献

已发表文献

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[2] Zhu M, Zhang P, Yu S, et al. Targeting ZFP64/GAL-1 axis promotes therapeutic effect of nab-paclitaxel and reverses immunosuppressive microenvironment in gastric cancer. J Exp Clin Cancer Res. 2022;41(1):14. Published 2022 Jan 7. doi:10.1186/s13046-021-02224-x(IF:11.161)

[3] Zhang HT, Gui T, Liu RX, et al. Sequential targeting of YAP1 and p21 enhances the elimination of senescent cells induced by the BET inhibitor JQ1. Cell Death Dis. 2021;12(1):121. Published 2021 Jan 25. doi:10.1038/s41419-021-03416-1(IF:8.469)

[4] Chen YJ, Hong WF, Liu ML, et al. An integrated bioinformatic investigation of mitochondrial solute carrier family 25 (SLC25) in colon cancer followed by preliminary validation of member 5 (SLC25A5) in tumorigenesis. Cell Death Dis. 2022;13(3):237. Published 2022 Mar 14. doi:10.1038/s41419-022-04692-1(IF:8.469)

[5] Xun Y, Zhou P, Yang Y, et al. Role of Nox4 in High Calcium-Induced Renal Oxidative Stress Damage and Crystal Deposition. Antioxid Redox Signal. 2022;36(1-3):15-38. doi:10.1089/ars.2020.8159(IF:8.401)

[6] Yu W, Zong S, Zhou P, et al. Cochlear Marginal Cell Pyroptosis Is Induced by Cisplatin via NLRP3 Inflammasome Activation. Front Immunol. 2022;13:823439. Published 2022 Apr 20. doi:10.3389/fimmu.2022.823439(IF:7.561)

[7] Duan X, Huang Y, Chen X, et al. Moderate DNA hypomethylation suppresses intestinal tumorigenesis by promoting caspase-3 expression and apoptosis. Oncogenesis. 2021;10(5):38. Published 2021 May 4. doi:10.1038/s41389-021-00328-9(IF:7.485)

[8] Huang Y, Ma J, Meng Y, et al. Exposure to Oxadiazon-Butachlor causes cardiac toxicity in zebrafish embryos. Environ Pollut. 2020;265(Pt A):114775. doi:10.1016/j.envpol.2020.114775(IF:6.793)

[9] Qiang W, Shen T, Noman M, et al. Fibroblast Growth Factor 21 Augments Autophagy and Reduces Apoptosis in Damaged Liver to Improve Tissue Regeneration in Zebrafish. Front Cell Dev Biol. 2021;9:756743. Published 2021 Oct 22. doi:10.3389/fcell.2021.756743(IF:6.684)

[10] Wu Y, Zhang J, Li C, et al. The Activation of ROS/NF-κB/MMP-9 Pathway Promotes Calcium-Induced Kidney Crystal Deposition. Oxid Med Cell Longev. 2021;2021:8836355. Published 2021 Jun 8. doi:10.1155/2021/8836355(IF:6.543)

[11] Jiang S, Zhang Z, Huang F, et al. Protective Effect of Low Molecular Weight Peptides from Solenocera crassicornis Head against Cyclophosphamide-Induced Nephrotoxicity in Mice via the Keap1/Nrf2 Pathway. Antioxidants (Basel). 2020;9(8):745. Published 2020 Aug 13. doi:10.3390/antiox9080745(IF:6.313)

[12] Yang P, Yang Y, He X, et al. miR-153-3p Targets βII Spectrin to Regulate Formaldehyde-Induced Cardiomyocyte Apoptosis. Front Cardiovasc Med. 2021;8:764831. Published 2021 Dec 15. doi:10.3389/fcvm.2021.764831(IF:6.050)

[13] Chen YJ, Song HY, Zhang ZW, Chen Q, Tang ZP, Gu M. Extracts of Vine Tea Improve Diet-Induced Non-Alcoholic Steatohepatitis Through AMPK-LXRα Signaling. Front Pharmacol. 2021;12:711763. Published 2021 Jul 30. doi:10.3389/fphar.2021.711763(IF:5.811)

[14] Gong F, Shen T, Zhang J, et al. Nitazoxanide induced myocardial injury in zebrafish embryos by activating oxidative stress response. J Cell Mol Med. 2021;25(20):9740-9752. doi:10.1111/jcmm.16922(IF:5.310)

[15] Zhu D, Wang Z, Zhang G, et al. Periostin promotes nucleus pulposus cells apoptosis by activating the Wnt/β-catenin signaling pathway. FASEB J. 2022;36(7):e22369. doi:10.1096/fj.202200123R(IF:5.192)

[16] Xu H, Qu C, Gan L, et al. Deletion of the Impg2 gene causes the degeneration of rod and cone cells in mice. Hum Mol Genet. 2020;29(10):1624-1634. doi:10.1093/hmg/ddaa062(IF:5.101)

[17] Li Q, Qin M, Tan Q, et al. MicroRNA-129-1-3p protects cardiomyocytes from pirarubicin-induced apoptosis by down-regulating the GRIN2D-mediated Ca
2+ signalling pathway. J Cell Mol Med. 2020;24(3):2260-2271. doi:10.1111/jcmm.14908(IF:4.486)

[18] Wen Y, Zong S, Liu T, Du P, Li H, Xiao H. Tauroursodeoxycholic acid attenuates cisplatin-induced ototoxicity by inhibiting the accumulation and aggregation of unfolded or misfolded proteins in the endoplasmic reticulum. Toxicology. 2021;453:152736. doi:10.1016/j.tox.2021.152736(IF:4.221)

[19] Meng Y, Yin Q, Ma Q, et al. FXII regulates the formation of deep vein thrombosis via the PI3K/AKT signaling pathway in mice. Int J Mol Med. 2021;47(5):87. doi:10.3892/ijmm.2021.4920(IF:4.101)

[20] Jin S, Yang L, Meng C, et al. Sequential Epiphyseal Cartilage Changes of Femoral Heads in C57BL/6 Female Mice Treated with Excessive Glucocorticoids. Cartilage. 2021;13(2_suppl):453S-464S. doi:10.1177/1947603520978574(IF:3.857)

[21] Feng Y, Lei B, Zhang H, et al. MicroRNA-136-5p from Endothelial Progenitor Cells-released Extracellular Vesicles Mediates TXNIP to Promote the Dissolution of Deep Venous Thrombosis. Shock. 2022;57(5):714-721. doi:10.1097/SHK.0000000000001920(IF:3.454)

[22] Wang H, He F, Liang B, et al. p53-Dependent LincRNA-p21 Protects Against Proliferation and Anti-apoptosis of Vascular Smooth Muscle Cells in Atherosclerosis by Upregulating SIRT7 via MicroRNA-17-5p. J Cardiovasc Transl Res. 2021;14(3):426-440. doi:10.1007/s12265-020-10074-9(IF:3.312)

[23] Liu Q, Xu X, Sun W. Down-regulated HSA_circ_0003528 inhibits hepatocellular carcinoma aggressiveness via the miR-212-3p/XIAP axis. Bioengineered. 2022;13(4):11269-11280. doi:10.1080/21655979.2022.2066046(IF:3.269)

[24] Xu J, Zhang B, Chu Z, Jiang F, Han J. Wogonin Alleviates Cisplatin-induced Cardiotoxicity in Mice Via Inhibiting Gasdermin D-mediated Pyroptosis. J Cardiovasc Pharmacol. 2021;78(4):597-603. Published 2021 Jun 16. doi:10.1097/FJC.0000000000001085(IF:3.105)

[25] Wang S, Zeng X, Yang Y, et al. Hypothermic oxygenated perfusion ameliorates ischemia-reperfusion injury of fatty liver in mice via Brg1/Nrf2/HO-1 axis. Artif Organs. 2022;46(2):229-238. doi:10.1111/aor.14076(IF:3.094)

[26] Xie WJ, Hou G, Wang L, Wang SS, Xiong XX. Astaxanthin suppresses lipopolysaccharide‑induced myocardial injury by regulating MAPK and PI3K/AKT/mTOR/GSK3β signaling. Mol Med Rep. 2020;22(4):3338-3346. doi:10.3892/mmr.2020.11443(IF:2.952)

[27] Zhang Y, Li Q, Xu D, et al. Idarubicin-induced oxidative stress and apoptosis in cardiomyocytes: An in vitro molecular approach. Hum Exp Toxicol. 2021;40(12_suppl):S553-S562. doi:10.1177/09603271211033774(IF:2.903)

[28] Li Q, Qin M, Li T, et al. Rutin protects against pirarubicin-induced cardiotoxicity by adjusting microRNA-125b-1-3p-mediated JunD signaling pathway. Mol Cell Biochem. 2020;466(1-2):139-148. doi:10.1007/s11010-020-03696-9(IF:2.795)

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