MTT Cell Proliferation Assay Kit MTT细胞增殖及细胞毒性检测试剂盒
勾选需要的规格可一次提交;也可以逐个询价。货号会一并带入询价单。
Q:MTT 配置成工作液后的使用注意事项?
A:MTT 配制成溶液后为黄色,需避光保存,长时间光照会导致失效。当颜色变为灰绿色时, 请勿使用。MTT 溶液在 4℃、冰浴等较低温度情况下会凝固,可以 20-25℃水浴温育片刻至全部融解 后使用。
Q:配好的 MTT 溶液是否需要过滤?
A:无需过滤。
已发表文献
[1] Wei Q, Arami H, Santos HA, et al. Intraoperative Assessment and Photothermal Ablation of the Tumor Margins Using Gold Nanoparticles. Adv Sci (Weinh). 2021;8(5):2002788. Published 2021 Jan 18. doi:10.1002/advs.202002788(IF:16.806)
[2] Zhong D, Chen W, Xia Z, et al. Aggregation-induced emission luminogens for image-guided surgery in non-human primates. Nat Commun. 2021;12(1):6485. Published 2021 Nov 10. doi:10.1038/s41467-021-26417-2(IF:14.919)
[3] Zhang D, Zhong D, Ouyang J, et al. Microalgae-based oral microcarriers for gut microbiota homeostasis and intestinal protection in cancer radiotherapy. Nat Commun. 2022;13(1):1413. Published 2022 Mar 17. doi:10.1038/s41467-022-28744-4(IF:14.919)
[4] Zhong D, Li W, Hua S, et al. Calcium phosphate engineered photosynthetic microalgae to combat hypoxic-tumor by in-situ modulating hypoxia and cascade radio-phototherapy. Theranostics. 2021;11(8):3580-3594. Published 2021 Jan 22. doi:10.7150/thno.55441(IF:11.556)
[5] Li Y, Chen W, Qi Y, et al. H2 S-Scavenged and Activated Iron Oxide-Hydroxide Nanospindles for MRI-Guided Photothermal Therapy and Ferroptosis in Colon Cancer. Small. 2020;16(37):e2001356. doi:10.1002/smll.202001356(IF:11.459)
[6] Wang X, Qin X, Yan M, et al. Loss of exosomal miR-3188 in cancer-associated fibroblasts contributes to HNC progression. J Exp Clin Cancer Res. 2019;38(1):151. Published 2019 Apr 8. doi:10.1186/s13046-019-1144-9(IF:11.161)
[7] Pan X, Qi Y, Du Z, et al. Zinc oxide nanosphere for hydrogen sulfide scavenging and ferroptosis of colorectal cancer. J Nanobiotechnology. 2021;19(1):392. Published 2021 Nov 27. doi:10.1186/s12951-021-01069-y(IF:10.435)
[8] Zhong D, Zhao J, Li Y, et al. Laser-triggered aggregated cubic α-Fe2O3@Au nanocomposites for magnetic resonance imaging and photothermal/enhanced radiation synergistic therapy. Biomaterials. 2019;219:119369. doi:10.1016/j.biomaterials.2019.119369(IF:10.273)
[9] Fan X, Luo Z, Chen Y, et al. Oxygen self-supplied enzyme nanogels for tumor targeting with amplified synergistic starvation and photodynamic therapy. Acta Biomater. 2022;142:274-283. doi:10.1016/j.actbio.2022.01.056(IF:8.947)
[10] Jiang Z, Wang T, Yuan S, et al. A tumor-sensitive biological metal-organic complex for drug delivery and cancer therapy. J Mater Chem B. 2020;8(32):7189-7196. doi:10.1039/d0tb00599a(IF:5.344)
[11] Sun X, Su F, Luo X, Ning Y. The Use of Bionic Prodrugs for the Enhancement of Low Dose Radiotherapy. Front Chem. 2021;9:710250. Published 2021 Aug 11. doi:10.3389/fchem.2021.710250(IF:5.221)
[12] Yuan J, Li X, Zhang G, et al. USP39 mediates p21-dependent proliferation and neoplasia of colon cancer cells by regulating the p53/p21/CDC2/cyclin B1 axis. Mol Carcinog. 2021;60(4):265-278. doi:10.1002/mc.23290(IF:4.784)
[13] Hong Z, Pan J, Chen M, et al. Long Intergenic Noncoding RNA 00641 Promotes Growth and Invasion of Colorectal Cancer through Regulating miR-450b-5p/GOLPH3 Axis. J Oncol. 2022;2022:8259135. Published 2022 Jun 15. doi:10.1155/2022/8259135(IF:4.375)
[14] Zhang J, Li P, Li T, Zhou Z, Wu H, Zhang L. Coronin 6 promotes hepatocellular carcinoma progression by enhancing canonical Wnt/beta-catenin signaling pathway. J Cancer. 2021;12(24):7465-7476. Published 2021 Nov 4. doi:10.7150/jca.62873(IF:4.207)
[15] Shi H, Chi H, Luo Z, et al. Self-Healable, Fast Responsive Poly(ω-Pentadecalactone) Thermogelling System for Effective Liver Cancer Therapy. Front Chem. 2019;7:683. Published 2019 Oct 18. doi:10.3389/fchem.2019.00683(IF:3.782)
[16] Jiang JZ, Yang BH, Ji LL, et al. Metabolic-induced cytotoxicity of diosbulbin B in CYP3A4-expressing cells. Toxicol In Vitro. 2017;38:59-66. doi:10.1016/j.tiv.2016.11.006(IF:3.338)
[17] Zhang R, Li F, Wang W, Wang X, Li S, Liu J. The effect of antisense inhibitor of miRNA 106b∼25 on the proliferation, invasion, migration, and apoptosis of gastric cancer cell. Tumour Biol. 2016;37(8):10507-10515. doi:10.1007/s13277-016-4937-x(IF:2.926)
[18] Wang N, Zeng GZ, Yin JL, Bian ZX. Artesunate activates the ATF4-CHOP-CHAC1 pathway and affects ferroptosis in Burkitt's Lymphoma. Biochem Biophys Res Commun. 2019;519(3):533-539. doi:10.1016/j.bbrc.2019.09.023(IF:2.705)
[19] Huan Q, Cheng SC, Du ZH, Ma HF, Li C. LncRNA AFAP1-AS1 regulates proliferation and apoptosis of endometriosis through activating STAT3/TGF-β/Smad signaling via miR-424-5p. J Obstet Gynaecol Res. 2021;47(7):2394-2405. doi:10.1111/jog.14801(IF:1.730)
[20] Liu D, Yuan R, Yang F, Zhang D. Effects of tanshinones mediated by forkhead box O3a transcription factor on the proliferation and apoptosis of lung cancer cells. Oncol Lett. 2019;17(1):450-455. doi:10.3892/ol.2018.9530(IF:1.664)
CCK-8,科研实验中最常见的细胞增殖检测方法
技术讲解
Bio-Firefly荧光素酶报告基因检测应用——ADCC细胞毒性检测实验方案
应用方向
ADCC检测效应细胞株,助力药物开发
应用方向
细胞增殖与毒性常用的检测方法
技术讲解