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Q:这个 BrdU 怎么用，需要 37℃加热吗？
A:需要 37℃加热
Q:冰冻切片的 BrdU 染色，不出结果，满片子都是非特异染色，胞浆着色？
A:可能是灌注时没有灌干净，红细胞留在组织里，最后就是掌握显色时间。
Q：用于动物和细胞上，然后一抗二抗染色无荧光信号？
A：封闭前需要使用2N盐酸处理1h，然后洗去盐酸，再加入等量的0.1M Na2B4O7 室温浸泡15min，洗去四硼酸钠。目的是：2N盐酸可以使DNA双链变性解开，暴露存在DNA双链中的抗原，即充分暴露BRDU； Na2B4O7 中和盐酸的酸化，若不进行中和会引起后期抗体效价下降，从而导致染色结果不好或者染不出来。
Published citations
[1] Wang X, Wang HY, Hu GS, et al. DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity. Cell Rep. 2021;35(12):109281. doi:10.1016/j.celrep.2021.109281(IF:9.423)
[2] Cao Z, Xiao Q, Dai X, et al. circHIPK2-mediated σ-1R promotes endoplasmic reticulum stress in human pulmonary fibroblasts exposed to silica. Cell Death Dis. 2017;8(12):3212. Published 2017 Dec 13. doi:10.1038/s41419-017-0017-4(IF:5.965)
[3] Zhao SJ, Shen YF, Li Q, et al. SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway. Cell Death Dis. 2018;9(3):390. Published 2018 Mar 9. doi:10.1038/s41419-018-0419-y(IF:5.638)
[4] Chen L, Luo W, Zhang W, et al. circDLPAG4/HECTD1 mediates ischaemia/reperfusion injury in endothelial cells via ER stress. RNA Biol. 2020;17(2):240-253. doi:10.1080/15476286.2019.1676114(IF:5.477)
[5] Chu H, Wang W, Luo W, et al. CircHECTD1 mediates pulmonary fibroblast activation via HECTD1. Ther Adv Chronic Dis. 2019;10:2040622319891558. Published 2019 Nov 27. doi:10.1177/2040622319891558(IF:4.455)
[6] Cui S, Li F. RHPN1‑AS1 promotes ovarian carcinogenesis by sponging miR‑6884‑5p thus releasing TOP2A mRNA. Oncol Rep. 2021;46(4):221. doi:10.3892/or.2021.8172(IF:3.906)
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