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Product data sheet PD-96476ES
Recombinant SARS-COV-2 Spike S1 Protein,hFc Tag Recombinant SARS-COV-2 Spike S1 Protein,hFc Tag
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Recombinant SARS-COV-2 Spike S1 Protein,hFc Tag

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新冠研究
Product no.96476ES Sizes3 options SourceYeasen · Official
Cat. No. 96476ES25
现货 ¥1,295.00
Qty 1
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Cat.No Spec Price Status
96476ES25 25 μg ¥1,295.00 现货 Enquire
96476ES60 100 μg ¥5,185.00 现货 Enquire
96476ES76 500 μg ¥17,255.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No96476ES
Specification高活性、高纯度、低内毒素、高批间一致性
Origin翌圣生物科技(上海)股份有限公司
应用方法 新冠研究研究SARS-CoV-2 S蛋白与ACE2受体的结合
适用物种种属 SARS-CoV-2
检测方法 ELISA SPR SDS-PAGE LAL
生物特性参数 蛋白类型Spike S1 protein 种属来源SARS-CoV-2 生物活性描述EC50 of 59.7ng/ml for binding to Human ACE2 by ELISA; affinity constant of 0.132 μM to Human ASGR1 by SPR 应用ELISA Data : Immobilized Human ACE2 His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike S1 hFc Tag with the EC50 of 59.7ng/ml determined by ELISA .SPR Data: Biotinylated Human ASGR1 His Tag captured on CM5 Chip via Anti-his antibody can bind SARS-COV-2 Spike S1 hFc Tag with an affinity constant of 0.132 μM as determined in SPR assay .
技术参数 纯度> 95% as determined by SDS-PAGE 内毒素水平Less than 1 EU per μg by the LAL method 分子量The protein has a predicted MW of 101.6 kDa. Due to glycosylation the protein migrates to 115-130 kDa based on Bis-Tris PAGE result. 制剂形式Supplied as 0.22μm filtered solution in PBS (pH 7.4) 储存条件-85~-65℃保存,收到货之日起,有效期1年。建议分装蛋白为一次实验使用量,分装后,-80℃冻存。避免反复冻融。 表达系统HEK293 标签C-hFc 外观Liquid 表达序列Val16-Arg685 登录号QHO60594.1 产品别称S1 protein;Spike protein S1;Spike S1 protein;S glycoprotein Subunit1
实验类型 ELISA测定S1蛋白与ACE2受体的结合活性(EC50=59.7ng/ml) SPR测定S1蛋白与ASGR1受体的亲和力(KD=0.132μM) SDS-PAGE测定蛋白纯度与分子量

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