Recombinant Biotinylated Human OSMR beta Protein,Avi Tag

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HEK293细胞表达系统抗体药物靶点研究免疫学检测

Product no.96219ES Sizes3 options SourceYeasen · Official

Cat. No. 96219ES25

现货 $2,255.00

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96219ES25 25 μg $2,255.00 现货 Enquire

96219ES60 100 μg $9,005.00 现货 Enquire

96219ES76 500 μg $30,005.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96219ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 抗体药物靶点研究作为靶点蛋白用于抗体筛选与开发 免疫学检测用于ELISA等免疫检测中的蛋白标准品或捕获抗原

适用物种种属人

检测方法 ELISA SDS-PAGE HPLC Bis-Tris PAGE LAL法

生物特性参数 蛋白类型重组生物素化蛋白 种属来源人 生物活性ELISA Data 1: Immobilized Biotinylated Human OSMR beta Avi Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-OSMR Antibody hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. 应用抗体药物靶点研究；ELISA免疫检测

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE; > 90% as determined by HPLC 内毒素水平Less than 1 EU per μg by the LAL method. 分子量The protein has a predicted MW of 82.93 kDa. Due to glycosylation the protein migrates to 110-130 kDa based on Bis-Tris PAGE result. 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 储存条件-25~-15℃保存，收到货之日起，有效期1年。复溶后，-85~-65℃保存，3个月有效期。避免反复冻融。 活性ELISA 检测 EC50=3.6 ng/ml（Anti-OSMR Antibody hFc Tag） 表达系统HEK293 标签C-Avi 表达序列Glu28-Met740 外观冻干粉 蛋白登录号Q99650-1 复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg，建议复溶到0.5mg/mL的浓度。对于500μg或1mg的冻干粉，建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装，储存于至-80℃，避免反复冻融。