Recombinant Human PD-L1/B7-H1 Protein,hFc Tag

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HEK293表达系统

Product no.93708ES Sizes3 options SourceYeasen · Official

Cat. No. 93708ES60

现货 $3,385.00

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Cat.No Spec Price Status

93708ES60 100 μg $3,385.00 现货 Enquire

93708ES76 500 μg $11,305.00 现货 Enquire

93708ES25 25 μg $915.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93708ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

适用物种种属人

检测方法 ELISA SPR SDS-PAGE HPLC LAL

生物特性参数 蛋白类型重组蛋白 种属来源人 生物活性描述Immobilized Human PD-1 mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1 hFc Tag with the EC50 of 0.93μg/ml determined by ELISA; Human PD-L1 hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1 His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay. 表达系统HEK293 标签C-hFc AccessionQ9NZQ7-1 别名CD274 PDL1 PD-L1 B7-H1 PDCD1L1 PDCD1LG1

技术参数 内毒素水平<1.0 EU per μg (LAL method) 分子量预测52 kDa；糖基化后SDS-PAGE迁移至68-80 kDa 纯度>95% (SDS-PAGE and HPLC) 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4) containing 8% trehalose 储存条件-25~-15℃保存，有效期1年；复溶后-85~-65℃保存，3个月 表达序列Phe19-Arg238 复溶方法开盖前离心，用蒸馏水复溶，浓度>100 μg/mL，建议分装避免反复冻融 外观Lyophilized Powder 活性ELISA EC50=0.93 μg/ml; SPR KD=2.356 μM