Recombinant Human GITR/TNFRSF18 (His Tag)

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HEK293细胞表达系统免疫检查点研究ELISASPR

Product no.93554ES Sizes4 options SourceYeasen · Official

Cat. No. 93554ES20

现货 $985.00

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Cat.No Spec Price Status

93554ES20 20 μg $985.00 现货 Enquire

93554ES60 100 μg $3,585.00 现货 Enquire

93554ES76 500 μg $12,355.00 现货 Enquire

93554ES80 1 mg $24,155.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93554ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 免疫检查点研究研究领域 ELISA体外结合活性检测应用 SPR分子互作分析应用

适用物种种属人

检测方法 ELISA SPR SDS-PAGE HPLC LAL法

生物特性参数 蛋白类型重组蛋白/靶点蛋白/免疫检查点 种属来源人 生物活性描述1.【ELISA Data】Immobilized Human GITR His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer His Tag with the EC50 of 0.2μg/ml determined by ELISA. 2.【ELISA Data】Immobilized Human GITR His Tag at 0.5μg (100μl/Well) on the plate. Dose response curve for Anti-GITR Antibody hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. 3.【SPR Data】Human GITR His Tag captured on CM5 Chip via Anti-His Antibody can bind Human GITR Ligand Trimer hFc Tag with an affinity constant of 4.76 nM as determined in SPR assay. 应用产品数据Tris-Bis PAGE

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量The protein has a predicted MW of 15.4 kDa. Due to glycosylation the protein migrates to 25-28 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 活性ELISA: EC50=0.2 μg/ml (Biotinylated Human GITR Ligand Trimer); EC50=7.0 ng/ml (Anti-GITR Antibody); SPR: KD=4.76 nM (Human GITR Ligand Trimer). 表达系统HEK293 标签C-His 表达序列Gln26-Glu161 Accession编号Q9Y5U5-1 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 外观Lyophilized Powder 产品别称CD357 GITR GITR-D TNFRSF18 AITR