Recombinant Human CD27/TNFRSF7 (His Tag)

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HEK293表达系统免疫检查点研究淋巴细胞活化与存活研究B细胞免疫应答研究

Product no.93550ES Sizes4 options SourceYeasen · Official

Cat. No. 93550ES20

现货 $985.00

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93550ES20 20 μg $985.00 现货 Enquire

93550ES60 100 μg $4,445.00 现货 Enquire

93550ES76 500 μg $14,855.00 现货 Enquire

93550ES80 1 mg $28,225.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93550ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 免疫检查点研究研究CD27/CD70共刺激信号通路 淋巴细胞活化与存活研究支持CD4+ T、CD8+ T、记忆T和NK细胞的活化与存活研究 B细胞免疫应答研究用于生发中心形成、记忆B细胞扩增与亲和力成熟的机制研究

适用物种种属人

检测方法 ELISA SDS-PAGE HPLC 鲎试剂法（LAL）

生物特性参数 蛋白类型重组蛋白；免疫检查点蛋白（CD27/TNFRSF7，C-His标签） 种属来源人（Human） 生物活性描述【ELISA Data】Immobilized Human CD27 His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27 Ligand hFc Tag with the EC50 of 37.9ng/ml determined by ELISA. CD27 acts as a co-stimulatory molecule supporting lymphocyte activation and survival. 应用免疫检查点研究；淋巴细胞活化与存活研究；B细胞生发中心形成及记忆B细胞扩增与亲和力成熟研究

样本类型 重组蛋白

技术参数浓度建议复溶浓度超过100 μg/mL（冻干粉） 纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量The protein has a predicted MW of 18.1 kDa. Due to glycosylation the protein migrates to 40-60 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性Immobilized Human CD27 His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27 Ligand hFc Tag with the EC50 of 37.9ng/ml determined by ELISA. 储存条件存储温度：-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统HEK293 标签C-His 表达序列Thr21-Ile192 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 外观Lyophilized Powder AccessionP26842 产品别称CD27 antigen Tp55 TNFRSF7 S152 T14 LPFS2 MGC20393 S152 CD27L receptor