Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein,hFc Tag

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HEK293表达系统

Product no.93358ES Sizes1 options SourceYeasen · Official

Cat. No. 93358ES25 现货 $1,265.00

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93358ES25 25 μg $1,265.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93358ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

适用物种种属人

检测方法 酶联免疫吸附试验 SDS-聚丙烯酰胺凝胶电泳 高效液相色谱 鲎试剂法

生物特性参数 蛋白类型重组蛋白/靶点蛋白/抗体药物靶点蛋白 种属来源人 生物活性描述ELISA Data: Immobilized Human CD3E&CD3G hFc Tag at 0.5μg/ml dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1 hFc Tag with EC50 of 1.22μg/ml; Immobilized Human CD3E&CD3G hFc Tag at 1μg/ml dose response curve for Anti-CD3E&CD3G Ab.2 mFc Tag with EC50 of 20.7ng/ml.

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Predicted MW 37.8 kDa (CD3E) and 36.5 kDa (CD3G); migrates to 40-60 kDa by Tris-Bis PAGE due to glycosylation. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4) 5% trehalose added as protectant before lyophilization. 表达系统HEK293 标签C-hFc AccessionP07766(CD3E)&P09693(CD3G) 表达序列Asp23-Asp126(CD3E)&Gln23-Ser116(CD3G) 外观Lyophilized Powder 储存条件-25~-15℃保存，有效期1年；复溶后，无菌条件下-85~-65℃保存，有效期3个月。 复溶方法开盖前离心，使用蒸馏水复溶冻干蛋白，复溶浓度超过100 μg/mL，建议分装避免反复冻融。