Recombinant FITC-Labeled Human EGFRVIII Protein, His-Avi Tag

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HEK293表达系统CAR-T靶点研究SDS-PAGE流式细胞术ELISA

Product no.93206ES Sizes1 options SourceYeasen · Official

Cat. No. 93206ES60 现货 $8,485.00

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93206ES60 100 μg $8,485.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93206ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 CAR-T靶点研究作为EGFRvIII靶点抗原用于CAR-T疗法研发 SDS-PAGE蛋白分析 流式细胞术流式分析 ELISA抗体检测

适用物种种属人

检测方法 SDS-PAGE ELISA LAL法 流式细胞术

生物特性参数 蛋白类型荧光标记重组蛋白 种属来源人 (Human) 生物活性描述Immobilized FITC-Labeled Human EGFRVIII His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody hFc Tag with the EC50 of 1.2ng/ml determined by ELISA. 应用SDS-PAGE、FACS检测；CAR-T靶点研究

技术参数纯度>95% (SDS-PAGE法) 内毒素水平<1.0 EU/μg (LAL法) 分子量预测41.6 kDa；Tris-Bis PAGE迁移至68-80 kDa（糖基化） 制剂形式0.22 μm过滤溶液，PBS (pH 7.4) 活性ELISA EC50=1.2 ng/ml 储存条件-85~-65℃保存，有效期6个月；避免反复冻融，使用前离心，建议分装后-80℃冻存 表达系统HEK293 表达序列Leu25-Ser378 标签C-His-Avi 外观Liquid 荧光波长Excitation 490 nm; Emission 520 nm 蛋白登录号NP_001333870.1 别名ErbB EC 2.7.10 EC 2.7.10.1 EGFR mENA LEGFR ERBB ERBB1 HER1 PIG61 NISBD2