Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.
Q:10185(Mouse Tissue Direct PCR Kit With Dye)和 10181(Mouse Tissue Direct PCR Kit)样本裂解温度和裂解操作一样吗?
A:均不一样。样本裂解温度区别: 10185:95℃,10-30min。10181:65℃,10-30 min。
裂解操作区别:10185:裂解组分A 和终止裂解组分 B 分开加,终止裂解混匀即可。 10181:裂解组分A 和终止裂解组分B 一起加,但终止裂解需要 5min。
Q:小鼠组织直接pcr 是鼠趾还是鼠尾好?需不需要纯化?
A:不需要纯化,离心取上清(选做),没比较鼠尾还是鼠趾效果,一般都是用鼠尾
Q:这款产品PCR结束后是否可以直接上样?
A:可以的
Published citations
[1] Luo J, Yang Q, Zhang X, et al. TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022;185(6):980-994.e15. doi:10.1016/j.cell.2022.02.010(IF:41.584)
[2] Zhao J, Chen J, Li YY, Xia LL, Wu YG. Bruton's tyrosine kinase regulates macrophage‑induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021;48(3):177. doi:10.3892/ijmm.2021.5010(IF:4.101)
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