Recombinant Human FOLR1 (His-Avi Tag)

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SDS-PAGEHPLCTris-Bis PAGECAR-T研究肿瘤研究叶酸代谢研究

Product no.93107ES Sizes4 options SourceYeasen · Official

Cat. No. 93107ES20

现货 $985.00

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93107ES20 20 μg $985.00 现货 Enquire

93107ES60 100 μg $3,585.00 现货 Enquire

93107ES76 500 μg $12,355.00 现货 Enquire

93107ES80 1 mg $24,155.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93107ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 SDS-PAGE HPLC Tris-Bis PAGE

应用方法 CAR-T研究靶点蛋白 肿瘤研究癌症相关研究 叶酸代谢研究叶酸摄取机制研究

适用物种种属 Human

检测方法 ELISA LAL法

生物特性参数 蛋白类型重组蛋白/靶点蛋白 种属来源Human 生物活性描述FOLR1介导细胞摄取叶酸和还原型叶酸，在早期胚胎发育中必需。ELISA测定的EC50为11.9 ng/mL。 应用CAR-T靶点蛋白，用于肿瘤免疫治疗研究。

样本类型 重组蛋白

技术参数纯度>95% as determined by SDS-PAGE and HPLC. 内毒素水平<1.0 EU per μg by the LAL method. 分子量Approximately 27.5 kDa. Due to glycosylation the protein migrates to 38-45 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性ELISA Data: Immobilized Human FOLR1 His Tag at 0.5μg/mL(100μL/Well) on plate. Dose response curve for Anti-FOLR1 Antibody hFc Tag with the EC50 of 11.9ng/mL determined by ELISA. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达序列Arg25-Met233 表达系统HEK293 标签C-His-Avi 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 AccessionP15328 外观Lyophilized Powder