Recombinant Sirius Protein 重组深蓝色荧光蛋白

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荧光成像双FRET

Product no.92661ES Sizes3 options SourceYeasen · Official

Cat. No. 92661ES10

现货 $535.00

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Cat.No Spec Price Status

92661ES10 10μg $535.00 现货 Enquire

92661ES60 100μg $1,945.00 现货 Enquire

92661ES80 1 mg $8,815.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No92661ES

Purity> 98% as determined by SDS-PAGE.

Specification10μg

Origin翌圣生物科技(上海)股份有限公司

应用方法 荧光成像用于检测细胞内钙变化、成像细菌和监测蛋白结构变化 双FRET同时跟踪钙动态和蛋白相互作用

生物特性参数 蛋白类型重组荧光蛋白 生物活性高活性；发射峰424 nm；pH 3-9稳定；可用于检测细胞内钙变化、成像细菌和监测蛋白结构变化 应用检测细胞内钙变化、成像细菌、监测蛋白结构变化、双FRET

技术参数纯度> 98% as determined by SDS-PAGE 内毒素水平< 0.01 EU/μg (LAL method) 分子量Approximately 26.7 kDa (239 amino acids) 制剂形式Lyophilized from 0.2 μm filtered solution in 20 mM PB 150 mM NaCl pH 7.4 外观Lyophilized Powder 标签No Tag 表达系统E.coli 储存条件-25 ~ -15℃保存，有效期1年；复溶后2~8℃保存7天；-85 ~ -65℃保存3个月 复溶方法开盖之前，先离心，以将内容物带到底部。使用无菌蒸馏水复溶，重新配制至0.1-1.0 mg/mL的浓度，可根据后续实验更进一步稀释；长期储存，建议稀释缓冲液中加入载体蛋白(0.1% BSA)；分装为一次实验用量，-80℃冻存，避免反复冻融。 发射峰424 nm pH稳定性pH 3-9 结构特征11股β-桶结构 表达序列MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLQFGVLCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNGISSNVYITADKQKNGIKAHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLESVTAAGITLGMDELYK