Recombinant Protein L (Freeze-Dried) 重组蛋白L（冻干）

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大肠杆菌表达系统抗体纯化

Product no.20546ES Sizes4 options SourceYeasen · Official

Cat. No. 20546ES03

现货 ¥855.00

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Cat.No Spec Price Status

20546ES03 1 mg ¥855.00 现货 Enquire

20546ES10 10 mg ¥1,885.00 现货 Enquire

20546ES25 25 mg ¥3,165.00 现货 Enquire

20546ES60 100 mg ¥7,915.00 现货 Enquire

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Specifications

Brand翌圣生物 Yeasen

Cat.No20546ES

Purity> 96% as determined by SDS-PAGE and HPLC.

Specification1 mg

Origin翌圣生物科技(上海)股份有限公司

复溶方法 蒸馏水或生理盐水溶解

Accession D6S9W1

生物活性 The recombinant Protein L is a genetically engineered protein containing 5 IgG-binding regions of protein L. Cell wall binding region cell membrane binding region and albumin binding region have been removed from the recombinant Protein L to ensure the maximum specific IgG binding. The recombinant Protein L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein L binds to human mouse rat and pig IgG.

分子量 Approximately 40.6 kDa with a Cys on C-terminus a single non-glycosylated polypeptide chain containing 366 amino acids. But it migrates with an apparent molecular mass of 46 kDa in SDS-PAGE.

外观 Lyophilized Powder

制剂 无添加剂，冻干

纯度 > 96% as determined by SDS-PAGE and HPLC.

表达序列 KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAGC

280 nm处1个单位DO的浓度 1.132 mg/ml

标签 No Tag

内毒素 < 0.1 EU per µg by the LAL method.

表达系统 E.coli

技术平台 大肠杆菌表达系统

应用方法 抗体纯化利用Protein L与IgG kappa轻链结合的特性，纯化多克隆或单克隆IgG抗体

检测方法 SDS-PAGE HPLC LAL法

生物特性参数 蛋白类型重组蛋白 生物活性描述重组蛋白L包含5个kappa结合结构域，能通过kappa轻链相互作用结合抗体，不干扰抗体-抗原结合位点。用于纯化多克隆或单克隆IgG抗体，可与人、小鼠、大鼠和猪IgG结合。应用常用于纯化多克隆或单克隆IgG抗体，可与人、小鼠、大鼠和猪IgG结合。 结合种属人、小鼠、大鼠、猪 结合结构域5个kappa结合结构域 去除区域细胞壁结合区、细胞膜结合区和白蛋白结合区

技术参数纯度> 96% as determined by SDS-PAGE and HPLC. 内毒素水平< 0.1 EU per µg by the LAL method. 分子量Approximately 40.6 kDa; apparent molecular mass of 46 kDa in SDS-PAGE. 制剂形式无添加剂，冻干 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统E.coli 标签No Tag 外观Lyophilized Powder 复溶方法蒸馏水或生理盐水溶解 280 nm处1个单位OD的浓度1.132 mg/ml 氨基酸数366 糖基化非糖基化