HiActi® Recombinant Human R spondin 3/RSPO3 Protein,His Tag 重组人RSPO3蛋白

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HEK293细胞表达系统Wnt信号通路研究干细胞研究

Product no.92283ES Sizes3 options SourceYeasen · Official

Cat. No. 92283ES60

现货 $3,885.00

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92283ES60 100 μg $3,885.00 现货 Enquire

92283ES76 500 μg $11,655.00 现货 Enquire

92283ES25 25 μg $1,885.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No92283ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 Wnt信号通路研究研究Rspo/β-catenin信号通路 干细胞研究调节干细胞群增殖与分化研究

适用物种种属人

检测方法 SDS-PAGE ELISA SPR LAL法

生物特性参数 蛋白类型重组人R-Spondin 3蛋白/细胞因子 种属来源人 (Human) 生物活性描述ELISA: Immobilized Human R-Spondin 3 His Tag at 1μg/ml 与Human RNF43 hFc Tag结合的EC50为25.2ng/ml；SPRHuman LGR-5 hFc Tag与Human R-Spondin 3 His Tag结合的亲和常数为0.59 nM 应用Wnt/β-catenin信号通路研究；调节干细胞群增殖和分化研究 表达序列Gln22-Val146 产品别称R-spondin-3 RSPO3 hPWTSR hRspo3 PWTSR THSD2

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE 内毒素水平< 1.0 EU per μg by LAL method 分子量Predicted MW 14.95 kDa; migrates to 20-35 kDa due to glycosylation based on Tris-Bis PAGE 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4); normally 8% trehalose added as protectant before lyophilization 储存条件-25 ~ -15℃保存，收到货后有效期1年；复溶后 -85 ~ -65℃保存，3个月有效期 表达系统HEK293 标签C-His 蛋白登录号Q9BXY4-1 外观Lyophilized Powder 复溶方法开盖前先离心；在无菌蒸馏水中重新配制至0.1-1.0 mg/mL，可根据后续实验进一步稀释；避免反复冻融 活性ELISA: 与Human RNF43结合的EC50为25.2 ng/ml；SPR: 与Human LGR-5结合的亲和常数为0.59 nM