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Product data sheet PD-10102ES updated 2026-10-13
2× Hieff® PCR Master Mix (With Dye) 2× Hieff® PCR Master Mix (With Dye)
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2× Hieff® PCR Master Mix (With Dye)

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PCR高GC含量扩增常规PCR
Product no.10102ES Sizes4 options Documents1 files SourceYeasen · Official
Cat. No. 10102ES03
现货 $18.00 促$40.00
Qty 1
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Specifications & Enquiry

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Cat.No Spec Price Status
10102ES03 1 mL $18.00促$40.00 现货 Enquire
10102ES08 5× 1 mL $88.00促$150.00 现货 Enquire
10102ES50 50× 1 mL $798.00促$1,500.00 现货 Enquire
10102ES60 100× 1 mL $1,498.00促$3,000.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No10102ES
Specification1 mL
Application常规PCR
Shelf Life2年
Origin翌圣生物科技(上海)股份有限公司
技术平台 PCR
应用方法 高GC含量扩增扩增高GC含量基因 常规PCR常规DNA扩增
适用物种种属 人 拟南芥
检测方法 琼脂糖凝胶电泳
生物特性参数 试剂类型PCR预混液 应用常规PCR、高GC含量扩增
样本类型 基因组DNA
技术参数 浓度2× 缓冲液组分Taq DNA Polymerase dNTPs MgCl2 优化缓冲体系 储存条件-25~-15℃保存,有效期2年 延伸时间30 s/kb 退火温度Tm-(2~5)℃ 产品形式预混液 末端结构3'-dA 扩增长度≤5 kb GC范围40-70% 电泳指示剂蓝色 稳定性4℃放置3个月,-20℃放置1年,25℃放置1个月
实验类型 常规PCRDNA扩增

FAQ

Q:PCR Mix 可以用于 PAGE 电泳吗?

A:不建议,PCR Mx 中含有影响 PAGE 胶电泳的组分。推荐客户使用 10101

Q:PCR Mix 可以减少体系么?

A:可以,使用的引物、模板等比减少使用。

Q:10102 和 10103 有什么区别?

A:区别在于 10102 预混有溴酚蓝指示剂。后续可直接进行凝胶电泳。而 10103 中无溴酚蓝指示剂,电泳时需要添加DNA loading buffer。

Q:扩增完送测序。应选有染料的还是无染料?

A:都可以。PCR 产物测序,测序公司会先进行纯化。

Q:10102 镁离子浓度,dNTP 浓度分别是多少?

A:镁离子终浓度 2mM,200 µM of each dNTP。

Q:PCR Mix 扩增完成后,可以直接做酶切吗?

A:不建议,某些组分可能会影响酶切效果。

Q:PCR Mix 有没有 3’-5’的外切酶活性?PCR Mix 扩增产物类型?

A:没有 3’-5’的外切酶活性,产物带有 3’-A。可用于TA 克隆。

Documents & Citations

Published citations

[1] Wu LY, Shang GD, Wang FX, et al. Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration. Dev Cell. 2022;57(4):526-542.e7. doi:10.1016/j.devcel.2021.12.019(IF:12.270)

[2] Li Y, Wang C, Zhang L, Chen B, Mo Y, Zhang J. Claudin-5a is essential for the functional formation of both zebrafish blood-brain barrier and blood-cerebrospinal fluid barrier. Fluids Barriers CNS. 2022;19(1):40. Published 2022 Jun 3. doi:10.1186/s12987-022-00337-9(IF:7.662)

[3] Luo T, Wang Y, Tang H, et al. An AAV-Based NF-κB-Targeting Gene Therapy (rAAV-DMP-miR533) to Inflammatory Diseases. J Inflamm Res. 2022;15:3447-3466. Published 2022 Jun 14. doi:10.2147/JIR.S362732(IF:6.922)

[4] Chen Z, Qi Z, He D, et al. Strategy for Scanning Peptide-Coding Circular RNAs in Colorectal Cancer Based on Bioinformatics Analysis and Experimental Assays. Front Cell Dev Biol. 2022;9:815895. Published 2022 Feb 25. doi:10.3389/fcell.2021.815895(IF:6.684)

[5] Yu Y, Fang L. CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer. Cell Death Discov. 2022;8(1):152. Published 2022 Apr 2. doi:10.1038/s41420-022-00965-y(IF:5.241)

[6] Zhang J, Liu W, Li G, et al. BCAS2 is involved in alternative splicing and mouse oocyte development. FASEB J. 2022;36(2):e22128. doi:10.1096/fj.202101279R(IF:5.192)

[7] Xiao W, Li J, Zhang Y, et al. A fungal Bipolaris bicolor strain as a potential bioherbicide for goosegrass (Eleusine indica) control. Pest Manag Sci. 2022;78(3):1251-1264. doi:10.1002/ps.6742(IF:4.845)

[8] Zhang Y, Yu R, Tang J, et al. Three cytochrome P450 CYP4 family genes regulated by the CncC signaling pathway mediate phytochemical susceptibility in the red flour beetle, Tribolium castaneum. Pest Manag Sci. 2022;78(8):3508-3518. doi:10.1002/ps.6991(IF:4.845)

[9] Zhang X, Yang S, Chen W, et al. Circular RNA circYPEL2: A Novel Biomarker in Cervical Cancer. Genes (Basel). 2021;13(1):38. Published 2021 Dec 23. doi:10.3390/genes13010038(IF:4.096)

[10] Gu K, Qian D, Qin H, et al. A novel mutation in KCNH2 yields loss-of-function of hERG potassium channel in long QT syndrome 2. Pflugers Arch. 2021;473(2):219-229. doi:10.1007/s00424-021-02518-1(IF:3.657)

[11] Yang Y, Chu X, Nie M, et al. A novel long-range deletion spanning STX16 and NPEPL1 causing imprinting defects of the GNAS locus discovered in a patient with autosomal-dominant pseudohypoparathyroidism type 1B. Endocrine. 2020;69(1):212-219. doi:10.1007/s12020-020-02304-6(IF:3.235)

[12] Ullah H, Arbab S, Khan MIU, et al. Circulating cell-free mitochondrial DNA fragment: A possible marker for early detection of Schistosoma japonicum. Infect Genet Evol. 2021;88:104683. doi:10.1016/j.meegid.2020.104683(IF:2.773)

[13] Xu L, Chen Y, Shen T, Lin C, Zhang B. Genetic Analysis of PICK1 Gene in Alzheimer's Disease: A Study for Finding a New Gene Target. Front Neurol. 2019;9:1169. Published 2019 Jan 9. doi:10.3389/fneur.2018.01169(IF:2.635)

[14] Ullah H, Qadeer A, Giri BR. Detection of circulating cell-free DNA to diagnose Schistosoma japonicum infection. Acta Trop. 2020;211:105604. doi:10.1016/j.actatropica.2020.105604(IF:2.555)

[15] Yan ZC, Hua HQ, Qi GY, Li YX. Early Detection and Identification of Parasitoid Wasps Trichogramma Westwood (Hymenoptera: Trichogrammatidae) in Their Host Eggs Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism [published online ahead of print, 2022 Jun 24]. J Econ Entomol. 2022;toac095. doi:10.1093/jee/toac095(IF:2.381)

[16] Zhou Z, Yin H, Suye S, Zhu F, Cai H, Fu C. The changes of DNA double-strand breaks and DNA repair during ovarian reserve formation in mice. Reprod Biol. 2022;22(1):100603. doi:10.1016/j.repbio.2022.100603(IF:2.376)

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