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Product data sheet PD-10154ES updated 2026-10-13
2× Hieff Canace® Plus PCR Master Mix (With Dye) 高保真酶预混液 2× Hieff Canace® Plus PCR Master Mix (With Dye) 高保真酶预混液
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2× Hieff Canace® Plus PCR Master Mix (With Dye) 高保真酶预混液

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PCR高保真PCR长片段PCR
Product no.10154ES Sizes3 options Documents1 files SourceYeasen · Official
Cat. No. 10154ES01
现货 $105.00
Qty 1
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Specifications & Enquiry

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Cat.No Spec Price Status
10154ES01 100 μL $105.00 现货 Enquire
10154ES03 1 mL $148.00促$585.00 现货 Enquire
10154ES08 5× 1 mL $700.00促$1,655.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No10154ES
Specification100 μL
Application高保真PCR
Shelf Life1年
Origin翌圣生物科技(上海)股份有限公司
技术平台 PCR
应用方法 高保真PCR用于高保真扩增DNA片段 长片段PCR用于扩增长达13 kb的DNA片段
适用物种种属 人 小鼠 水稻
检测方法 琼脂糖凝胶电泳
生物特性参数 试剂类型高保真酶预混液 应用高保真PCR 酶活性5'→3' DNA聚合酶活性;3'→5'核酸外切酶活性
样本类型 基因组DNA cDNA 质粒DNA
技术参数 浓度2× 缓冲液组分优化的缓冲体系(含dNTPs、两种单克隆抗体) 储存条件-25~-15℃保存,有效期1年 保真度83×(相对Taq酶) 延伸时间30 sec/kb(极限10 sec/kb) 退火温度68℃ 产品形式Mix 末端结构平末端 扩增长度人gDNA/cDNA≤10kb;λDNA≤13kb GC范围30%-60% 电泳指示剂是 热启动是 灵敏度1 pg模板 稳定性37°C保存7 d,反复冻融50次不影响性能 扩增速度10 sec/kb(极限)
实验类型 PCR扩增聚合酶链式反应扩增DNA 热启动PCR利用热启动DNA聚合酶提高反应特异性

FAQ

Q:不同模板的推荐使用量是多少?

A:基因组DNA:50–200 ng(扩增1–10 kb片段);质粒/病毒DNA:10 pg–20 ng;cDNA:1–2.5 μL(不超过总反应体积的1/10)

Q:预混液反复冻融会影响性能吗?

A:产品含特异保护剂,反复冻融后仍能保持稳定活性,但仍建议分装保存以减少冻融次数。

Documents & Citations

Published citations

[1] Zhao S, Zhang Y, Tan M, et al. Identification of YABBY Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera. Plants (Basel). 2023;12(2):380.doi:10.3390/plants12020380(IF:4.658)

[2] Zhang Z, Liu X, Tao M, et al. Taxonomy, Phylogeny, Divergence Time Estimation, and Biogeography of the Family Pseudoplagiostomataceae (Ascomycota, Diaporthales). J Fungi (Basel). 2023;9(1):82. doi:10.3390/jof9010082(IF:5.724)

[3] Chen L, Xu ZL, Liu PG, et al. Identification of Three Viruses Infecting Mulberry Varieties. Viruses. 2022;14(11):2564. doi:10.3390/v14112564(IF:5.818)

[4] Wang W, Shinwari KI, Zhang H, et al. The bHLH Transcription Factor OsbHLH057 Regulates Iron Homeostasis in Rice. Int J Mol Sci. 2022;23(23):14869. doi:10.3390/ijms232314869(IF:6.208)

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