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Product data sheet PD-90369ES
HiActi® Recombinant Human IL-33 Protein 重组人白介素-33 HiActi® Recombinant Human IL-33 Protein 重组人白介素-33
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HiActi® Recombinant Human IL-33 Protein 重组人白介素-33

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大肠杆菌表达系统免疫学研究细胞信号传导研究
Product no.90369ES Sizes5 options SourceYeasen · Official
Cat. No. 90369ES80
现货 ¥22,245.00
Qty 1
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Cat.No Spec Price Status
90369ES80 1 mg ¥22,245.00 现货 Enquire
90369ES60 100 μg ¥4,825.00 现货 Enquire
90369ES05 2 μg ¥535.00 现货 Enquire
90369ES10 10 μg ¥985.00 现货 Enquire
90369ES50 50 μg ¥2,955.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No90369ES
Specification高纯度、高活性、低内毒素、高批间一致性。
Origin翌圣生物科技(上海)股份有限公司
技术平台 大肠杆菌表达系统
应用方法 免疫学研究IL-33参与Th2免疫反应,用于炎症与免疫机制研究 细胞信号传导研究IL-33/ST2L信号通路研究
适用物种种属 人
检测方法 SDS-PAGE HPLC LAL法 细胞增殖实验
生物特性参数 蛋白类型重组蛋白/细胞因子 种属来源Human 生物活性描述Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng/ml corresponding to a specific activity of > 2.0 × 10^7 IU/mg. 应用炎症与免疫研究、细胞信号传导研究
样本类型 重组蛋白
技术参数 纯度>97% by SDS-PAGE and HPLC 内毒素水平Less than 1 EU/µg of rHuIL-33 as determined by LAL method 分子量Approximately 17.9 kDa a single non-glycosylated polypeptide chain containing 159 amino acids 制剂形式Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB 150 mM NaCl 1mM EDTA pH7.4 储存条件-25 ~ -15℃保存,收到货之后有效期1年;复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期 活性ED50 < 0.05 ng/ml corresponding to a specific activity of > 2.0 × 10^7 IU/mg 表达系统E.coli 表达序列Ser112-Thr270 标签No Tag 外观Lyophilized Powder AccessionO95760 复溶方法开盖之前,先离心,以将内容物带到底部。使用无菌蒸馏水复溶,重新配制至0.1-1.0 mg/mL的浓度,可根据后续实验更进一步稀释;长期储存,建议稀释缓冲液中加入载体蛋白(0.1% BSA);分装为一次实验用量,-80℃冻存,避免反复冻融
实验类型 细胞增殖实验测定ED50和比活性

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