HiActi® Recombinant Mouse IL-34 Protein ,His Tag 重组小鼠白介素-34

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Product no.90297ES Sizes4 options SourceYeasen · Official

Cat. No. 90297ES08

现货 $885.00

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Cat.No Spec Price Status

90297ES08 5 μg $885.00 现货 Enquire

90297ES20 20 μg $2,215.00 现货 Enquire

90297ES60 100 μg $6,885.00 现货 Enquire

90297ES76 500 μg $19,885.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90297ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

应用方法通用研究应用

适用物种种属小鼠

检测方法 SDS-PAGE LAL法

生物特性参数 蛋白类型白细胞介素-34 (IL-34) 种属来源小鼠 生物活性描述Measure by its ability to induce human peripheral blood monocytes (PBMCs) proliferation. The ED₅₀ for this effect is <30 pg/mL. 应用在髓系细胞的增殖、存活、分化和迁移中发挥重要作用，由IL-34激活ERK1/2、JAK和PI3K/AKT信号通路介导。

技术参数 内毒素水平<0.1 EU per 1 μg of the protein by the LAL method. 分子量Calculated MW of 26.17 kDa; migrates as 30 kDa under reducing condition 纯度>98% as determined by SDS-PAGE. 制剂形式The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS pH 8.0. 表达系统E.coli 标签C-His 外观Lyophilized Powder 复溶方法1. Open tube centrifuge at 3000 rpm for 5 min. 2. Reconstitute the lyophilized powder in sterile water to a concentration not less than 100 μg/mL and let stand for at least 20 min to dissolve. 3. The reconstituted solution can be further diluted and aliquoted. For further dilution add carrier protein (0.1% BSA 10% FBS or 5% HSA); for serum-free experiments replace with 5% trehalose solution as carrier. 储存条件-25 ~ -15°C for 1 year after receipt. After reconstitution 2 ~ 8°C for 7 days; -85 ~ -65°C for 3 months. It is recommended to aliquot upon first use to avoid repeated freeze-thaw cycles. 表达序列MNEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVPSPQSCSPEPSLQYAATQLYPPPPWSPSSPPHSTGSVRPVRAQGEGLLP with polyhistidine tag at the C-terminus. AccessionQ6ZMJ4 活性ED50 <30 pg/mL (induce human PBMCs proliferation)