HiActi® Recombinant Mouse IL-31 Protein ,His Tag 重组小鼠白介素-31

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大肠杆菌表达系统免疫学研究细胞生物学研究血液学研究细胞因子研究

Product no.90296ES Sizes4 options SourceYeasen · Official

Cat. No. 90296ES08

现货 $885.00

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90296ES08 5 μg $885.00 现货 Enquire

90296ES20 20 μg $2,215.00 现货 Enquire

90296ES60 100 μg $6,885.00 现货 Enquire

90296ES76 500 μg $19,885.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90296ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 大肠杆菌表达系统

应用方法 免疫学研究炎症与免疫应答机制研究 细胞生物学研究细胞增殖机制研究 血液学研究造血调控研究 细胞因子研究细胞因子诱导及信号通路研究

适用物种种属小鼠

检测方法 SDS-PAGE LAL法 细胞增殖实验

生物特性参数 蛋白类型细胞因子/白介素/IL-31 种属来源小鼠 生物活性描述Measured by its ability to induce A549 cells proliferation. The ED₅₀ for this effect is < 40 ng/mL 应用细胞增殖、造血、细胞因子诱导、炎症和免疫应答；促进细胞介导免疫 受体IL-31RA 信号通路MAPK、PI3K/AKT、Jak/STAT 分泌细胞Th2细胞 产品别称interleukin 31 IL31

样本类型 重组蛋白

技术参数纯度>98% as determined by SDS-PAGE 内毒素水平<0.1 EU per 1 μg of the protein by the LAL method 分子量计算MW 16.78 kDa；还原条件下SDS-PAGE约18 kDa 制剂形式The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS pH 8.0 活性Measured by its ability to induce A549 cells proliferation. The ED₅₀ for this effect is < 40 ng/mL 储存条件-25 ~ -15℃保存，有效期1年；复溶后2~8℃保存7天；-85~-65℃保存3个月；建议分装避免重复冻融 表达系统E.coli 标签C-His AccessionQ6EBC2 外观Lyophilized Powder 复溶方法3000 rpm离心5分钟；无菌水重悬至浓度不低于100 μg/mL，静置至少20 min；进一步稀释可加0.1%BSA、10%FBS或5%HSA；无血清可用5%海藻糖 表达序列MSHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT with polyhistidine tag at the C-terminus 氨基酸残基数141