Alkaline Phosphatase Activity Detection Kit 碱性磷酸酶活性检测试剂盒
勾选需要的规格可一次提交；也可以逐个询价。货号会一并带入询价单。
Q：为什么该试剂盒检测不准？
A：可能是试剂盒各个成分没有恢复到室温即使用。
Q：在结果检测时 405nm 处测不到吸光值，怎么办？
A：如果不能测定 405nm，也可以在 400-415nm 范围内检测吸光度。如果不能立即测定，可以在数小时内完成测定，所显现的黄色在数小时（≤ 6h）内稳定。
Q：37℃孵育 5min，为什么检测不出来酶活？
A：建议延长孵育时间至 30min。
Q：该试剂盒中的产品可以直接在室温下操作的吗？
A：ALP 反应缓冲液和 p-nitrophenol 标准品溶液对人体有害，请注意适当防护。反应终止液有腐蚀性，请小心操作。最好在通风橱里操作。
Q：该试剂盒按说明书检测完后如何计算酶活？
A：首先，绘制标曲：0.5mM*标准品的用量分别为4、8、16、24、32和40微升，用微摩尔数做横坐标，标曲方程可通过Excel做；然后，根据标准曲线及待检测样品的吸光度值，可以计算得到样品中p-nitrophenol生成量。再根据酶活性定义进行计算。即p-nitrophenol的微摩尔数除以孵育时间（min）。计算出的酶活单位用U表示。最后，样品的酶活浓度可以用酶活/（上样体积*蛋白浓度），最后可以表示成U/mg。
已发表文献
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