5× SDS-PAGE Protein Loading Buffer 5× SDS-PAGE蛋白上样缓冲液

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SDS-PAGE蛋白电泳

Product no.20315ES Sizes3 options Documents1 files SourceYeasen · Official

Cat. No. 20315ES05

现货 $28.00

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20315ES05 2× 1 mL $28.00 现货 Enquire

20315ES20 20× 1 mL $225.00 现货 Enquire

20315ES76 500 mL $3,855.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No20315ES

Specification\

Origin翌圣生物科技(上海)股份有限公司

技术平台 SDS-PAGE

应用方法 蛋白电泳蛋白样品上样

生物特性参数应用蛋白电泳

技术参数浓度5×浓缩 制剂配方含溴酚蓝、SDS和DTT 储存条件-20℃储存，一年有效期 运输条件冰袋运输

实验类型 SDS-PAGE蛋白样品上样

FAQ

Q：该产品有沉淀，可以直接使用吗？

A：5×SDS-PAGE 蛋白上样缓冲液必须完全溶解后再使用。

Q：该产品有沉淀，能直接煮沸溶解吗？

A：不可以。在室温或不超过 37°C 的水浴中溶解 5×SDS-PAGE 上样缓冲液。水浴溶解后立即室温存放，尽量避免长时间置于水浴中。

Q：蛋白自身的电荷会对缓冲液有影响吗？

A：不会的。5×SDS-PAGE 蛋白上样缓冲液中 SDS 可与蛋白质结合使蛋白质－SDS 复合物上带有大量的负电荷，这时蛋白质本身的电荷完全被 SDS 掩盖，因此，电泳速度只是与其分子量大小有关。

Q：上样过程需要添加指示剂吗？

A：需要，一般采用溴酚蓝用作电泳指示剂，可大概指示电泳结束的时间。

Documents & Citations

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Published citations

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