Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.
Q:该 RIPA 裂解液的裂解程度?
A:胞膜、胞浆、胞核成分均强裂解。
Q:该裂解液的主要应用在哪些实验?
A:WB、IP。
Q:该裂解液会降解蛋白吗?
A:主要成分为 1% Triton X-100,1% deoxycholate,0.1% SDS,均为蛋白抑制剂, 可有效抑制蛋白降解。
Q:使用完该产品后,裂解产物中出现一小团透明胶状物,这是什么原因?如何去除?
A:RIPA 裂解液的裂解产物中经常会出现一小团透明胶状物,属正常现象。该透明胶状物为含有基因组DNA 等复合物。在不检测和基因组 DNA 结合特别紧密的蛋白的情况下,可以直接离心取上清用于后续实验;如果需要检测和基因组结合特别紧密的蛋白, 则可以通过超声处理打碎打散该透明胶状物,随后离心,取上清用于后续实验。
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