HisSep Ni-NTA Agarose Resin 6FF His标签蛋白琼脂糖高速纯化树脂

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亲和层析蛋白纯化

Product no.20503ES Sizes4 options Documents1 files SourceYeasen · Official

Cat. No. 20503ES10

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20503ES10 10 mL $758.00促~~$798.00~~ 现货 Enquire

20503ES50 50 mL $3,188.00促~~$3,358.00~~ 现货 Enquire

20503ES60 100 mL $5,548.00促~~$5,838.00~~ 现货 Enquire

20503ES80 1000 mL $44,686.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No20503ES

Specification基质（Matrix）高度交联的6%琼脂糖凝胶粒径（Bead size）45-165 µm载量（Capacity）＞40 mg 6×His-tagged protein/mL基质耐压（Tolerance Pressuremax）0.3 MPa

Origin翌圣生物科技(上海)股份有限公司

技术平台 亲和层析

应用方法 蛋白纯化His标签蛋白的纯化应用

生物特性参数 蛋白类型His标签蛋白 应用蛋白纯化

技术参数基质高度交联的6%琼脂糖凝胶 粒径45-165 µm 载量>40 mg 6×His-tagged protein/mL 基质耐压0.3 MPa 储存缓冲液含20%乙醇的1×PBS 储存条件4℃保存，有效期2年 运输条件冰袋运输 配体NTA 螯合离子Ni2+

实验类型 亲和层析用于His标签蛋白的分离纯化

FAQ

Q：为什么填料会呈现褐色？

A：缓冲液中含有DTT 等还原剂，适当降低还原剂 DTT 的浓度，或改用巯基乙醇。

Q：为什么在上样过程中蛋白发生沉淀？

A：可能是操作温度太低，建议在可在室温下进行上样。

Q：洗脱组分不纯（含多种蛋白）是什么原因？

A：可能是洗杂不彻底：建议增加Wash Buffer 体积。

Q：为什么洗脱组分中无目的蛋白？

A：可能是目的蛋白结合比较弱，在洗杂步骤中已被洗下来：提高 Wash Buffer 的pH 值，或者降低咪唑浓度。

Documents & Citations

蛋白纯化产品目录 Open document

Published citations

[1] Li C, Zhan W, Yang Z, et al. Broad neutralization of SARS-CoV-2 variants by an inhalable bispecific single-domain antibody. Cell. 2022;185(8):1389-1401.e18. doi:10.1016/j.cell.2022.03.009(IF:41.584)

[2] Li X, Liang Y, Lian C, et al. CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. Theranostics. 2021;11(20):9821-9832. Published 2021 Oct 11. doi:10.7150/thno.62187(IF:11.556)

[3] Zhang Z, Chu Y, Li C, et al. Anti-PEG scFv corona ameliorates accelerated blood clearance phenomenon of PEGylated nanomedicines. J Control Release. 2021;330:493-501. doi:10.1016/j.jconrel.2020.12.047(IF:7.727)

[4] Zhang X, Tang W, Wen H, et al. Evaluation of CTB-sLip for Targeting Lung Metastasis of Colorectal Cancer. Pharmaceutics. 2022;14(4):868. Published 2022 Apr 15. doi:10.3390/pharmaceutics14040868(IF:6.321)

[5] Wu Q, Huang Y, Gu L, Chang Z, Li GM. OTUB1 stabilizes mismatch repair protein MSH2 by blocking ubiquitination. J Biol Chem. 2021;296:100466. doi:10.1016/j.jbc.2021.100466(IF:5.157)