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Product data sheet PD-41211ES
Hieff® Lysis Buffer for Exosome 外泌体专用裂解液 Hieff® Lysis Buffer for Exosome 外泌体专用裂解液
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Hieff® Lysis Buffer for Exosome 外泌体专用裂解液

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外泌体研究蛋白定量
Product no.41211ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 41211ES20 现货 $355.00
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41211ES20 20 mL $355.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No41211ES
Specification使用说明1.实验准备:实验前先将裂解液2~8 ℃融化混匀后置于冰上;2.将外泌体样本与裂解液按体积比1:1混匀后置于冰上裂解10 min;3.于4 ℃以11,200×g(~12,000 rpm*)离心5 min取上清,用BCA法测定蛋白浓度
Origin翌圣生物科技(上海)股份有限公司
应用方法 外泌体研究提取外泌体蛋白用于后续分析 蛋白定量用于BCA法测定蛋白浓度
检测方法 BCA法 Western Blot
样本类型 外泌体
技术参数 产品组成Tris (pH7.4) NaCl Triton X-100 sodium deoxycholate SDS EDTA PMSF Protease Inhibitor Cocktail(AEBSF Aprotinin Bestatin E-64 Leupeptin Pepstatin A) 储存条件-20 ℃存储,有效期1年 运输条件冰袋运输 使用说明与外泌体样本1:1混匀,冰上裂解10 min,4 ℃ 11 200×g离心5 min取上清 注意事项含有高浓度去垢剂,不适用于Bradford法测定蛋白浓度;裂解后蛋白浓度应小于2 μg/μL
实验类型 蛋白裂解裂解外泌体蛋白 Western Blot检测外泌体蛋白表达

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