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产品资料 PD-40728ES
DAPI核酸染料 DAPI染液(5mg/ml) DAPI核酸染料 DAPI染液(5mg/ml)
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DAPI核酸染料 DAPI染液(5mg/ml)

DAPI Solution DAPI染液(5 mg/mL)

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荧光显微镜流式细胞仪细胞核染色细胞生物学微生物检测分子生物学
产品编号40728ES 可选规格1 种 来源翌圣生物 · 官网
产品编号 40728ES03 现货 ¥188.00 促¥198.00
数量 1
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货号 规格 价格 状态
40728ES03 1 mg(200 μL) ¥188.00促¥198.00 现货 询价
提交询价 价格为协议价,提交后由业务确认;大包装可申请试用装。

产品概述

技术参数

品牌翌圣生物 Yeasen
货号 / 型号40728ES
规格中文名称(Chinese Synonym)4’,6-联脒-2-苯基吲哚二盐酸盐英文名称(English Synonym)4’,6-Diamidino-2-phenylindole, dihydrochlorideCAS号(CAS NO.)2
产地翌圣生物科技(上海)股份有限公司
技术平台 荧光显微镜 流式细胞仪
应用方法 细胞核染色核酸染料 细胞生物学多色荧光标记 微生物检测DNA染色 分子生物学核酸染色
检测方法 荧光显微镜观察 流式细胞仪检测
生物特性参数 靶分子双链DNA、RNA 结合位点DNA富含AT序列的小沟 荧光颜色蓝色 染色机理与双链DNA富含AT序列的小沟结合,也可嵌入RNA的AU序列
样本类型 细胞 核酸
技术参数 浓度5 mg/mL 储存条件-20ºC避光保存,1年有效 运输条件冰袋运输 CAS号28718-90-3 分子式C16H17Cl2N5·2HCl 分子量350.25 荧光光谱DAPI的Ex/Em=340 nm/488 nm; DAPI-DNA的Ex/Em=358 nm/461 nm 产品形式溶液
实验类型 细胞凋亡检测复染剂 RNA原位杂交核酸染色 免疫检测复染剂 多色荧光标记蓝色荧光染料

常见问题

Q:  DAPI是一种好的活细胞核标记探针吗?
A:  DAPI被认为是一种半渗透性/无渗透性的细胞核染料。对细胞核的染色性能好坏依赖于细胞系类型。某些细胞系可用DAPI染色,某些完全不可以,而某些标记结果不一致。替代的话,建议用Hoechst 33342或Hoechst 33258,具有同DAPI一样的波长和核酸结合模式,但是有非常好的细胞膜渗透性。

Q.DAPI和Hoechst染料相当类似,如何二选一?
A:DAPI是非常通用的蓝色荧光染料,用于细胞核复染。对固定和透化的细胞/组织,具有非常明亮的细胞核标记。然而,此染料被认为是半渗透至不渗透的染料,用于活细胞染色的结合不一致。Hoechst 33342是一种细胞膜渗透性的染料,具有同DAPI的结合机制和荧光特征。非常适合用于活细胞成像,效果就如同DAPI用于固定细胞染色。

文档与文献

已发表文献

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