Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.
Q:染完细胞膜后,可以染细胞核吗?
A:可以。
Q:该系列探针是只标记细胞膜还是细胞所有的膜结构均会被染上颜色?
A:DiI/DiO/DiD 和 DiR 是一种亲脂性膜染料,进入细胞膜后可以侧向扩散,逐渐使含有膜结构的细胞均被染色。
Q:DiO 和 DiR 的区别?
A:荧光强度:DiO 染色荧光强度通常低于DiR;
固定细胞或组织:DiO 染色效果欠佳,DiR 染色效果较好;
荧光持续时间:DiR 持续时间较长。有反馈被 DiR 标记的肿瘤细胞在体外培养的条件下可以存活长达 4 周,在体内 15 天后依然有很强的荧光信号。
Q:先将细胞固定,再用 DiO 染色,发现细胞核和细胞质也全部染上,是什么原因?
A:有如下可能原因:
1.DiO 浓度过大,建议适当降低浓度;
2.DiO 染色时间过长,建议适当缩短时间;
3.使用其它不适当固定液会导致荧光背景较高,建议使用 4%多聚甲醛固定细胞;
4.产品购买时间过长,已过期;建议重新购买。
Q:使用DMSO配置DiO储存液时出现不能完全溶解的沉淀该如何处理?
A:按照说明书使用DMSO配置1~2 mM储存液时如出现明显不溶沉淀,需使用超声波助溶,条件参考:40kHz,50℃,时间1h左右。由于DiO在DMF中溶解度高于DMSO及乙醇,推荐使用DMF对DiO进行溶解,同样需要使用超声波助溶。
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