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产品资料 PD-40711ES
PI碘化丙啶 细胞核荧光染料 EB类似物 PI碘化丙啶 细胞核荧光染料 EB类似物
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PI碘化丙啶 细胞核荧光染料 EB类似物

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荧光显微镜共聚焦显微镜流式细胞仪荧光计细胞凋亡研究细胞周期研究荧光显微成像流式细胞分析
产品编号40711ES 可选规格2 种 来源翌圣生物 · 官网
产品编号 40711ES10
现货 ¥178.00 促¥189.00
数量 1
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货号 规格 价格 状态
40711ES10 10 mg ¥178.00促¥189.00 现货 询价
40711ES60 100 mg ¥1,098.00促¥1,155.00 现货 询价
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技术参数

品牌翌圣生物 Yeasen
货号 / 型号40711ES
规格10 mg
产地翌圣生物科技(上海)股份有限公司
技术平台 荧光显微镜 共聚焦显微镜 流式细胞仪 荧光计
应用方法 细胞凋亡研究活死细胞染色与凋亡鉴定 细胞周期研究检测DNA含量分析细胞周期 荧光显微成像荧光显微镜/共聚焦显微镜观察 流式细胞分析FL2通道检测核酸染色 多重荧光检测与抗体/探针联用复染 细胞核染色特异性染色细胞核
检测方法 荧光染色 荧光检测 流式细胞术 荧光显微成像 荧光分光光度法
生物特性参数 试剂类型细胞核荧光染料/核酸染料 靶标核酸DNA和RNA 作用机制嵌入DNA/RNA碱基对之间,无序列倾向性 结合比例约每4-5个DNA碱基对结合一个染料 荧光增强倍数与核酸结合后增强20-30倍 细胞膜通透性不穿透完整细胞膜,可染破损细胞 荧光特性结合核酸后Ex/Em=535/617 nm 斯托克斯位移较大,可同时检测核酸和荧光抗体 兼容染料Calcein-AM、Hoechst 33258/33342 流式检测通道FL2通道 细胞周期分析可用于PI单独染色检测细胞周期 活死细胞染色用于活细胞和死细胞染色鉴定 复染应用多重荧光染色复染剂 应用荧光显微镜、共聚焦、流式、荧光计
样本类型 细胞 组织 核酸
技术参数 CAS号25535-16-4 分子式C27H34I2N4 分子量668.4 外观暗红色结晶 纯度≥95% by HPLC 溶解性溶于水(1 mg/mL) 荧光光谱水溶液Ex/Em=493/636 nm;核酸结合Ex/Em=535/617 nm 最大激发波长游离493 nm;核酸结合535 nm 最大发射波长游离636 nm;核酸结合617 nm 激发光源氙气灯、泵弧灯或488 nm氩离子激光 储存条件4 ºC避光保存,至少一年稳定 运输条件冰袋(wet ice)运输
实验类型 细胞凋亡检测与Calcein-AM/Hoechst联用区分活死细胞 活/死细胞染色PI排斥活细胞,染色死细胞核 细胞周期分析PI单独染色检测细胞周期 多重荧光染色作为复染剂兼容多种荧光标记 免疫荧光兼容直接/间接荧光抗体检测 mRNA原位杂交用于原位杂交复染 组织染色用于组织切片核染色

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已发表文献

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