PI碘化丙啶染液(1mg/mL) 细胞核荧光染色液

Propidium iodide Staining Solution（1mg/mL）碘化丙啶染液（1mg/mL）

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荧光显微镜共聚焦显微镜流式细胞仪荧光计细胞凋亡研究细胞周期分析荧光显微成像流式细胞术

产品编号40710ES 可选规格1 种 来源翌圣生物 · 官网

产品编号 40710ES03 现货

数量 1

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货号规格价格状态

40710ES03 1 mL ¥258.00促~~¥265.00~~ 现货询价

提交询价价格为协议价，提交后由业务确认；大包装可申请试用装。

产品概述

技术参数

品牌翌圣生物 Yeasen

货号 / 型号40710ES

规格1 mL

产地翌圣生物科技(上海)股份有限公司

技术平台 荧光显微镜 共聚焦显微镜 流式细胞仪 荧光计

应用方法 细胞凋亡研究活细胞与死细胞染色和鉴定 细胞周期分析DNA含量检测与周期分布分析 荧光显微成像细胞核和核酸荧光成像 流式细胞术FL2通道检测核酸荧光信号 mRNA原位杂交多重荧光染色复染 组织染色组织切片细胞核复染

适用物种种属通用

检测方法 荧光检测 流式细胞分析 荧光显微成像

生物特性参数 试剂类型细胞核荧光染料/核酸染料 检测靶标DNA/RNA 作用机制嵌入碱基对与DNA/RNA结合，结合后荧光显著增强 结合比例约每4-5个DNA碱基对结合1个染料 膜通透性不能穿透完整细胞膜，可进入破损细胞膜 生物活性死细胞/破损细胞核染色，活细胞排斥 适用细胞系通用应用细胞凋亡、细胞周期、多重荧光染色复染

样本类型 细胞组织核酸

技术参数浓度1 mg/mL CAS号25535-16-4 分子式C27H34I2N4 分子量668.4 外观暗红色结晶 纯度≥95% by HPLC 溶解性溶于水（1 mg/mL） 荧光光谱水溶液Ex/Em=493/636 nm；核酸结合Ex/Em=535/617 nm 荧光增强与核酸结合后荧光增强20-30倍 波长迁移激发红移约30-40 nm，发射蓝移约15 nm 激发光源氙气灯、汞弧灯或488 nm氩离子激光 检测通道流式细胞仪FL2通道 产品形式水溶性PI储存液 工作液配制用合适缓冲液稀释至工作液 储存条件4°C避光保存，至少1年稳定 运输条件冰袋（wet ice）运输

实验类型 细胞凋亡检测与Calcein-AM/Hoechst等联用区分活死细胞 细胞周期分析PI单独染色检测细胞DNA含量 细胞核染色对破损细胞膜细胞核进行荧光染色 多重荧光染色作为复染剂兼容荧光抗体及原位杂交

常见问题

Q：想找红色染料，染死细胞的细胞核，有什么推荐？

A：40710 PI染液或者 40745 7-AAD染液都可以。

Q：为什么样本使用多聚甲醛，甲醛或者戊二醛固定，则需要进行RNase处理。样本用甲醇/醋酸或者丙酮固定，通常不需要此步操作？

A：甲醛、多聚甲醛戊二醛固定液固定原理是交联，酶在交联过程中会失活，若要去除RNA需要再加入RNase处理。甲醇醋酸丙酮固定原理是变性，但是对蛋白变性的作用轻，固定后可再溶解，所以不需要再加入RNase处理。

文档与文献

已发表文献

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