Recombinant Human CD73/NT5E Protein,His Tag

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HEK293细胞表达系统抗体药物靶点研究

Product no.93885ES Sizes3 options SourceYeasen · Official

Cat. No. 93885ES60

现货 $3,585.00

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Cat.No Spec Price Status

93885ES60 100 μg $3,585.00 现货 Enquire

93885ES76 500 μg $12,355.00 现货 Enquire

93885ES25 25 μg $1,195.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93885ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 抗体药物靶点研究作为靶点蛋白用于抗体药物研发

适用物种种属人

检测方法 SDS-PAGE 高效液相色谱 ELISA 鲎试剂法

生物特性参数 蛋白类型重组蛋白/靶点蛋白 种属来源人 生物活性描述ELISA Data: Immobilized Human CD73 His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD73 Antibody hFc Tag with the EC50 of 40.6ng/ml determined by ELISA. EnzymeActivity: Measured by its ability to hydrolyze the 5'-phosphate group from the Substrate adenosine-5'-monophosphate (AMP). The specific activity is >15000 pmol/min/μg 蛋白登录号P21589-1 产品别称CD73 NT5E NTE 5' nucleotidase (CD73) 5'-NT 5-NT E5NT EC 3.1.3.5 eN eNT NT NT55'-nucleotidase NT5 NTE CALJA

技术参数纯度> 95% as determined by SDS-PAGE and HPLC 内毒素水平< 1.0 EU per μg by the LAL method 分子量predicted MW 58.8 kDa; migrates to 60-70 kDa by Tris-Bis PAGE 制剂形式Lyophilized from 0.22μm filtered solution in 20mM Tris 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. 活性>15000 pmol/min/μg (specific activity) 储存条件-25 ~ -15℃保存，有效期1年；复溶后，无菌条件下，-85 ~ -65℃保存，3个月 表达序列Trp27-Lys547 标签C-His 表达系统HEK293 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。