CellTracker Green CMFDA (5-Chloromethylfluorescein Diacetate) 活细胞示踪剂CMFDA(绿色)
勾选需要的规格可一次提交;也可以逐个询价。货号会一并带入询价单。
Q:这些探针染细胞,有没有细胞特异性?会不会 293 细胞不好染,MSC 好染?
A:由于这些染料是亲脂性染料,是通过插入到脂质膜中进而被激光激发观察,对于动植物细胞没有特异性。
Q:这些探针荧光探针装载完多久后观察?
A:为减少各种可能的误差,尽量缩短探针装载后到仪器测定所用的时间(刺激时间除外)。
Q:这些探针染色细胞后,对细胞有毒性吗?
A:荧光探针类产品对活细胞都会有一定的毒性,因此建议在染色处理完成后应尽快完成检测和观察。
Q:为什么染色后发现荧光颜色较浅,应怎么操作进行改进?
A:可以尝试增加染色的时间和浓度。
Q:要看几个小时过程中活细胞的形态变化,应该怎么选?
A:通常使用亲脂性花青染料,如 DiI,DiO,DiD 或 DiR。
Q:染色液工作体积及对应染色细胞数量是否有推荐?
A:请参考对于200,000-1000,000个细胞请使用500 μL染色工作液。
已发表文献
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