品牌翌圣生物 Yeasen
货号 / 型号94008ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
适用物种种属
食蟹猴
检测方法
ELISA
SDS-PAGE
HPLC
LAL
生物特性参数
蛋白类型重组蛋白/细胞因子
种属来源Cynomolgus (食蟹猴)
生物活性描述Immobilized Cynomolgus GFRAL
His Tag at 1μg/ml on the plate; dose response curve for Cynomolgus GDF15
hFc Tag with EC503.6 ng/ml by ELISA
产品别称NAG-1
MIC-1
GDF-15
PLAB
AccessionG7PWZ3
技术参数
纯度>95% as determined by SDS-PAGE and HPLC
内毒素水平<1.0 EU per μg by LAL method
分子量predicted MW 37.9 kDa; migrates to 40-50 kDa by SDS-PAGE
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4)
8% trehalose as protectant
活性EC50=3.6 ng/ml determined by ELISA
储存条件-25 ~ -15℃; after reconstitution
-85 ~ -65℃ for 3 months
表达序列Ala197-Val308
表达系统HEK293
标签N-hFc
外观Lyophilized Powder
复溶方法Centrifuge before opening; reconstitute in distilled water at >100 μg/mL; aliquot to avoid repeated freeze-thaw
实验类型
ELISA生物活性检测
SDS-PAGE纯度鉴定
HPLC纯度分析
LAL内毒素检测