品牌翌圣生物 Yeasen
货号 / 型号94041ES
纯度> 95% as determined by SDS-PAGE and HPLC.
规格25 μg
产地翌圣生物科技(上海)股份有限公司
技术平台
Expi293表达系统
应用方法
COVID-19研究研究SARS-CoV-2 Spike蛋白与ACE2受体的结合及病毒入侵机制
适用物种种属
SARS-CoV-2
检测方法
SDS-PAGE凝胶电泳
高效液相色谱(HPLC)
酶联免疫吸附试验(ELISA)
鲎试剂法(LAL)
生物特性参数
蛋白类型重组蛋白/靶点蛋白/新冠相关蛋白
种属来源SARS-CoV-2
生物活性描述ELISA Data: Immobilized SARS-CoV-2 Spike RBD (Delta B.1.617.2)
His Tag at 1μg/ml on the plate. Dose response curve for Human ACE2
hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.
应用研究SARS-CoV-2 Spike蛋白与ACE2受体的相互作用；用于COVID-19相关研究
样本类型
重组蛋白
技术参数
纯度>95% as determined by SDS-PAGE and HPLC
内毒素水平<1.0 EU per 1μg of the protein by the LAL method
分子量Approximately 26.26 kDa; migrates to 35-40 kDa due to glycosylation
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4)
活性ELISA: EC50 of 9.8 ng/ml for binding to Human ACE2
表达系统Expi293 (HEK293 Cells)
标签His tag at C-terminal
表达区间Arg319-Phe541(L452R
T478K)
外观冻干粉
复溶方法Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve in distilled water.
Uniprot编号P0DTC2
Accession编号QHD43416.1
实验类型
ELISA检测蛋白与人ACE2的结合活性
SDS-PAGE检测蛋白纯度及分子量
HPLC检测蛋白纯度
LAL法检测内毒素水平
