品牌翌圣生物 Yeasen
货号 / 型号94046ES
纯度> 95% as determined by SDS-PAGE and HPLC
规格25 μg
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293细胞表达系统
适用物种种属
SARS-CoV-2
检测方法
SDS-PAGE
HPLC
LAL法
ELISA
生物特性参数
蛋白类型Recombinant SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein
种属来源SARS-CoV-2
生物活性描述ELISA Data: Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2)
His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2
hFc Tag with the EC50 of 85.6ng/ml determined by ELISA.
变异株Omicron BA.4/BA.5/BA.5.2
登录号QHD43416.1
Uniprot编号P0DTC2
表达区间Gln14-Arg683 with mutations (T19I
L24S
PPA25-27del
HV69-70del
G142D
V213G
G339D
S371F
S373P
S375F
T376A
D405N
R408S
K417N
N440K
L452R
S477N
T478K
E484A
F486V
Q498R
N501Y
Y505H
D614G
H655Y
N679K
P681H)
技术参数
纯度>95% as determined by SDS-PAGE and HPLC
内毒素水平<1.0 EU per 1μg of the protein by the LAL method
分子量Approximately 75.76 kDa; migrates to 100-120 kDa by SDS-PAGE due to glycosylation
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4)
活性EC50 of 85.6 ng/ml for binding to Human ACE2 by ELISA
表达系统HEK293 Cells (Expi293)
标签His tag at the C-terminal
外观冻干粉
复溶方法Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
实验类型
ELISA活性检测
