Biotinylated Recombinant Human ANGPT2/Angiopoietin-2 (His-Avi Tag)

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HEK293表达系统生物素标记His-Avi标签血管生成研究炎症研究淋巴管发育研究血管重塑研究

Product no.94153ES Sizes4 options SourceYeasen · Official

Cat. No. 94153ES25

现货 $2,285.00

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94153ES25 25 μg $2,285.00 现货 Enquire

94153ES60 100 μg $6,885.00 现货 Enquire

94153ES76 500 μg $24,115.00 现货 Enquire

94153ES80 1 mg $43,865.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No94153ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统 生物素标记 His-Avi标签

应用方法 血管生成研究ANGPT2参与血管生成调控 炎症研究ANGPT2参与炎症过程 淋巴管发育研究ANGPT2与Ang-1协同参与淋巴管发育 血管重塑研究ANGPT2是出生后血管重塑所必需

适用物种种属人

检测方法 SDS-PAGE Tris-Bis PAGE ELISA LAL法

生物特性参数 蛋白类型重组蛋白/靶点蛋白/抗体药物靶点蛋白 种属来源人 生物活性描述ELISA法检测Anti-ANGPT2 Antibody结合的EC50为6.7 ng/ml 应用产品数据；血管生成、炎症、淋巴管发育相关研究 天然存在形式还原条件下单体65-66 kDa；非还原条件下形成140 kDa二聚体、200 kDa三聚体、250-300 kDa四聚体/五聚体

样本类型 重组蛋白

技术参数浓度复溶浓度>100 μg/mL 纯度> 95% as determined by SDS-PAGE 内毒素水平< 1.0 EU per μg by the LAL method 分子量Approximately 28.4 kDa. Due to glycosylation the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性Immobilized Biotinylated Human ANGPT2 His Tag at 5μg/ml (100μl/Well) on the Streptavidin Procated plate. Dose response curve for Anti-ANGPT2 Antibody hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统HEK293 表达序列Lys275-Phe496 标签N-His-Avi 生物素标记是（Biotinylated） AccessionO15123.1 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 规格高纯度、高活性、低内毒素、高批间一致性