品牌翌圣生物 Yeasen
货号 / 型号94172ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
适用物种种属
Human
检测方法
SDS-PAGE
HPLC
LAL法
ELISA
Tris-Bis PAGE
生物特性参数
蛋白类型重组蛋白
种属来源Human
生物活性描述Immobilized Human NGAL
hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody
hFc Tag with the EC50 19.7ng/ml determined by ELISA.
技术参数
纯度> 95% as determined by SDS-PAGE and HPLC.
内毒素水平< 1.0 EU per μg by the LAL method.
分子量Predicted MW 47.2 kDa; due to glycosylation
migrates to 48-52 kDa by Tris-Bis PAGE.
制剂形式Lyophilized from 0.22μm filtered solution in 50mM MES
150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization.
活性EC50 19.7 ng/ml determined by ELISA.
储存条件-25 ~ -15℃保存，收到货之后有效期2年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。
表达系统HEK293
标签C-hFc
AccessionP80188-1
表达序列Gln21-Gly198
复溶方法Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES
150mM NaCl (pH 6.5).
外观Lyophilized Powder
实验类型
SDS-PAGE纯度鉴定
HPLC纯度鉴定
Tris-Bis PAGE分子量检测
LAL法内毒素检测
ELISA生物活性检测
HER2靶点蛋白：驱动肿瘤靶向治疗的核心力量
应用方向
细胞因子科学选型--常见问题解答
技术讲解
