品牌翌圣生物 Yeasen
货号 / 型号94190ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
适用物种种属
人
检测方法
SDS-PAGE
HPLC
ELISA
鲎试剂法
生物特性参数
蛋白类型Recombinant Human SECTM1 Protein (hFc Tag)
种属来源Human
生物活性描述【ELISA Data】Immobilized Human CD7
His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human SECTM1
hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
别名K-12
SECTM1
技术参数
纯度> 95% as determined by SDS-PAGE and HPLC.
内毒素水平< 1.0 EU per μg by the LAL method.
分子量The protein has a predicted MW of 39.4 kDa. Due to glycosylation
the protein migrates to 47-50 kDa based on Tris-Bis PAGE result.
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
活性【ELISA Data】Immobilized Human CD7
His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human SECTM1
hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。
表达序列Gln29-Gly145
表达系统HEK293
标签C-hFc
外观Lyophilized Powder
复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。
Accession编号AAH17716
实验类型
ELISA测定生物活性
SDS-PAGE测定纯度
HPLC测定纯度
鲎试剂法测定内毒素水平
HER2靶点蛋白：驱动肿瘤靶向治疗的核心力量
应用方向
细胞因子科学选型--常见问题解答
技术讲解
