Recombinant Human IL-2 R beta&IL-2 R gamma Protein,hFc Tag

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HEK293表达系统抗体药物靶点研究

Product no.94300ES Sizes3 options SourceYeasen · Official

Cat. No. 94300ES60

现货 ¥4,445.00

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94300ES60 100 μg ¥4,445.00 现货 Enquire

94300ES76 500 μg ¥14,855.00 现货 Enquire

94300ES25 25 μg ¥1,215.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No94300ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 抗体药物靶点研究靶点蛋白应用

适用物种种属人

检测方法 SDS-PAGE HPLC ELISA SPR

生物特性参数 蛋白类型重组蛋白 种属来源Human 生物活性描述ELISA: Immobilized Human IL-15RA&IL-15 His Tag at 2μg/ml (100μl/well) on the plate. Dose 21.5ng/ml determined by ELISA. SPR: Human IL-2 R beta&IL-2 R gamma hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2 No Tag with an affinity constant of 0.114 nM; bind Human IL-15Ra&IL-15 His Tag with an affinity constant of 0.084 nM. 应用抗体药物靶点研究

样本类型 重组蛋白

技术参数 表达系统HEK293 标签C-hFc 纯度>95% as determined by SDS-PAGE and HPLC 内毒素水平<1.0 EU per μg by the LAL method 分子量Predicted MW: 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma); Observed: 65-70 kDa&80-100 kDa (Tris-Bis PAGE) 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 外观Lyophilized Powder 复溶方法Centrifuge before opening. Reconstitute lyophilized protein in distilled water to a concentration over 100 μg/mL. Recommend aliquot to avoid repeated freeze-thaw cycles. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达序列Ala27-Thr240 (IL-2 R beta)&Leu23-Ala262 (IL-2 R gamma) AccessionP14784(IL-2 R beta)&P31785-1(IL-2 R gamma)