品牌翌圣生物 Yeasen
货号 / 型号94950ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
SDS-PAGE
ELISA
应用方法
重组蛋白作为靶点蛋白或抗体药物靶点蛋白用于研究
细胞因子GDF15重组蛋白，用于细胞信号通路及相关疾病研究
适用物种种属
大鼠
检测方法
LAL法
生物特性参数
蛋白类型重组蛋白/细胞因子
种属来源Rat
生物活性描述Immobilized Rat GDF-15
His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL
hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. Immobilized Rat GDF15
His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL
His Tag with the EC50 of 0.95μg/ml determined by ELISA.
应用高纯度、高活性、低内毒素、高批间一致性
产品别称NAG-1
MIC-1
GDF-15
PLAB
技术参数
纯度> 95% as determined by SDS-PAGE
内毒素水平< 1.0 EU per μg by the LAL method
分子量predicted MW 13.68 kDa; migrates to 15-17 kDa by SDS-PAGE
制剂形式Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9)
normally 8% trehalose added as protectant before lyophilization
活性ELISA: EC50 of 17.2ng/ml for Human GFRAL
hFc Tag; EC50 of 0.95μg/ml for Biotinylated Mouse GFRAL
His Tag
储存条件-25 ~ -15℃保存，有效期2年；复溶后，无菌条件下-85 ~ -65℃保存，3个月有效期
表达系统E.coli
标签N-His
外观Lyophilized Powder
复溶方法Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
AccessionQ9Z0J6
表达序列Ser189-Ala303
实验类型
ELISA用于测定生物活性，通过剂量反应曲线确定EC50
SDS-PAGE用于鉴定蛋白纯度及分子量
HER2靶点蛋白：驱动肿瘤靶向治疗的核心力量
应用方向
细胞因子科学选型--常见问题解答
技术讲解
