Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.
Q:配置PEI溶液的时候,不调PH是否可以?
A:可以不调。
Q:多配置一点母液冻存起来是否会延长效期?
A:不建议冻存,储存液4℃保存3个月。
Q:转染后是否需要换液?
A:若转染前进行了换液,可不进行换液,若需要换液可在转染后6-8h可以进行换液。
Q:转染的时候是否需要无血清培养基培养?
A:制备复合物的时候需要使用无血清培养基,加入细胞中的时候不要求无血清培养。
Q:转染形成复合物的时候过量加入的DNA和转染试剂的量比较多的时候有沉淀絮状物析出,为什么呢?
A:可能是DNA的纯度不是很纯,有杂质,某种物质引起的PEI析出浑浊,如果把DNA的量降下来会减少这种现象。不建议转染的时候加入过多的质粒和转染试剂,转染试剂效率较高不需要额外提高。
Q:10ml的PEI溶液大概用了多少氢氧化钠溶液呢?
A:客户提供的数据可参考,10ml大概需要400ul的氢氧化钠溶液。建议慢慢调,根据自己的配置情况而定,建议用10ul的枪头调PH,如果PH值过大,溶液浑浊了再调回6.8,可能会影响转染效率。
Q:PEI 40000是否可以用于单链RNA的转染?
A:不可以用于单链RNA的转染,会出现转染不成功的结果。
Published citations
[1] Luo J, Yang Q, Zhang X, et al. TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022;185(6):980-994.e15. doi:10.1016/j.cell.2022.02.010(IF:41.584)
[2] Chen Y, Luo R, Li J, et al. Intrinsic Radical Species Scavenging Activities of Tea Polyphenols Nanoparticles Block Pyroptosis in Endotoxin-Induced Sepsis [published correction appears in ACS Nano. 2022 Mar 3;:]. ACS Nano. 2022;16(2):2429-2441. doi:10.1021/acsnano.1c08913(IF:15.881)
[3] Chen ZH, Yan SM, Chen XX, et al. The genomic architecture of EBV and infected gastric tissue from precursor lesions to carcinoma. Genome Med. 2021;13(1):146. Published 2021 Sep 7. doi:10.1186/s13073-021-00963-2(IF:11.117)
[4] Huang G, Liu D, Wang W, et al. High-resolution structures of human Nav1.7 reveal gating modulation through α-π helical transition of S6IV. Cell Rep. 2022;39(4):110735. doi:10.1016/j.celrep.2022.110735(IF:9.423)
[5] Tian X, Liu L, Jiang W, Zhang H, Liu W, Li J. Potent and Persistent Antibody Response in COVID-19 Recovered Patients. Front Immunol. 2021;12:659041. Published 2021 May 28. doi:10.3389/fimmu.2021.659041(IF:7.561)
[6] Lin J, Chen Z, Yang L, et al. Cas9/AAV9-Mediated Somatic Mutagenesis Uncovered the Cell-Autonomous Role of Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 in Murine Cardiomyocyte Maturation. Front Cell Dev Biol. 2022;10:864516. Published 2022 Apr 1. doi:10.3389/fcell.2022.864516(IF:6.684)
[7] Nian F, Qian Y, Xu F, Yang M, Wang H, Zhang Z. LDHA promotes osteoblast differentiation through histone lactylation. Biochem Biophys Res Commun. 2022;615:31-35. doi:10.1016/j.bbrc.2022.05.028(IF:3.575)
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