Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.
Q:40187,40188,40189均是高浓度基质胶,化冻时间是多久?
A:把基质胶放入冰冰盒中,放入4度冰箱化冻3-4天左右,化冻期间可以摇晃混匀。
Q:40187,40188,40189应用方向?
A:主要应用于体内成瘤实验
Q:拿到的基质有色差的变化(淡黄色到深红色)是什么原因?
A:对于含酚红的基质胶主要原因是由于酚红和碳酸氢盐与CO2作用引起的,但与5%CO2平衡后色差即会减少。冻融后,轻轻摇晃试剂瓶使基质胶分散均匀。
Q:基质胶的操作要注意哪些事项?
A:所有操作需在无菌环境下进行,所涉及到到物品应预冷后进行操作。
Q:如融化后出现凝胶状或流动性不佳该如何处理?
A:如遇4℃冰上过夜解冻后基质胶未能呈现匀浆状(例如出现果冻状或半凝固状态,这是因高浓度基质胶特性所致),未呈现出较好流动性,同时枪头难以吸取,可使用预冷的DMEM培养基或预冷的PBS缓冲液进行稀释,另外可将枪头剪去前端一小段以助于吸取。
Published citations
[1] Liang, J., Bi, G., Huang, X., et al. (2025). CD24 is a promising immunotherapeutic target for enhancing efficacy of third-generation EGFR-TKIs on EGFR-mutated lung cancer. Cancer Communications, *45*(11), 1547-1578. doi: 10.1002/cac2.70068 (IF:24.9)
[2] Ren, W., Zheng, P., Zhou, Z., et al. (2025). Immunoglobulin-directed phase separation for cell-specific modulation of receptor signaling. Journal of the American Chemical Society, *147*(38), 34620-34631. doi: 10.1021/jacs.5c09699 (IF:15.7)
[3] Zhao, S., Liu, X., Luo, R., et al. (2025). USP38 functions as an oncoprotein by downregulating the p53 pathway through deubiquitination and stabilization of MDM2. Cell Death & Differentiation, *32*, 1128-1141. doi: 10.1038/s41418-025-01462-2 (IF:15.4)
[4] Hu, L., Ma, X., Cai, X., et al. (2026). The mitochondrial translocation of phosphorylated EZH2 promotes PARP inhibitor resistance in BRCA1-deficient epithelial ovarian cancer. Cell Discovery, *12*, 17. doi: 10.1038/s41421-026-00880-x (IF:13)
[5] Xiao, F., Zhu, H., Xiong, Y., Guo, Y., Zhang, Z., Zeng, J., Xiao, Y., Liao, B., Shang, X., Zhao, S., Hu, G., Huang, K., & Guo, H. (2024). Positive feedback loop of c-myc/XTP6/NDH2/NF-κB to promote malignant progression in glioblastoma. Journal of Experimental & Clinical Cancer Research, *43*(1), 187. doi: 10.1186/s13046-024-03109-5 (IF:11.4)
[6] Zhou, X., Wang, J., Lu, M., Fang, L., Zhao, J., & Li, D. (2025). Leucine-rich repeat-containing 56 promotes breast cancer progression via modulation of the RhoA/ROCKs signaling axis. Molecular Biomedicine, *6*(1), 31. doi: 10.1186/s43556-025-00271-w (IF:10.1)
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