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Q：绿光标记的siRNA，转染5h后观察，只有一些杂点，没有细胞形态什么原因？
A：转染进去的 整个细胞是绿色的，可以清晰的看到细胞形态，没有转染进去的就是绿色的杂点儿。
Q：转染后可以换液吗？
A：可以换液，转然后8h换液，建议不换液，转染试剂毒性很小，不换液转染效率可以提高。
Published citations
[1] Zhang Y, Hu R, Xi B, Nie D, Xu H, Liu A. Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells. Front Cell Dev Biol. 2022;10:829404. Published 2022 Mar 2. doi:10.3389/fcell.2022.829404(IF:6.684)
[2] Cui B, Wang Y, Jin J, et al. Resveratrol Treats UVB-Induced Photoaging by Anti-MMP Expression, through Anti-Inflammatory, Antioxidant, and Antiapoptotic Properties, and Treats Photoaging by Upregulating VEGF-B Expression. Oxid Med Cell Longev. 2022;2022:6037303. Published 2022 Jan 4. doi:10.1155/2022/6037303(IF:6.543)
[3] Liu Y, Zeng Y, Si HB, Tang L, Xie HQ, Shen B. Exosomes Derived From Human Urine-Derived Stem Cells Overexpressing miR-140-5p Alleviate Knee Osteoarthritis Through Downregulation of VEGFA in a Rat Model. Am J Sports Med. 2022;50(4):1088-1105. doi:10.1177/03635465221073991(IF:6.203)
[4] Zhang ZD, Wen B, Li DJ, et al. AKT serine/threonine kinase 2-mediated phosphorylation of fascin threonine 403 regulates esophageal cancer progression. Int J Biochem Cell Biol. 2022;145:106188. doi:10.1016/j.biocel.2022.106188(IF:5.085)
[5] Tan S, Zhou Y, Zhao H, et al. Comprehensive transcriptome analysis of hypothalamus reveals genes associated with disorders of sex development in pigs. J Steroid Biochem Mol Biol. 2021;210:105875. doi:10.1016/j.jsbmb.2021.105875(IF:4.294)
[6] Niu M, Yi M, Dong B, Luo S, Wu K. Upregulation of STAT1-CCL5 axis is a biomarker of colon cancer and promotes the proliferation of colon cancer cells. Ann Transl Med. 2020;8(15):951. doi:10.21037/atm-20-4428(IF:3.297)
