Hifair® V Reverse Transcriptase (600 U/μL, Glycerol-free)
勾选需要的规格可一次提交；也可以逐个询价。货号会一并带入询价单。
Q：这款产品有什么特点?
A：M-MLV (H-) Reverse Transcriptase 是通过对野生型 M-MLV 逆转录酶基因改造，在大肠杆菌中表达纯化而成。. 无 RNaseH 活性，具有合成能力高，热稳定性好和半衰期长的特点。
已发表文献
1. Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019, 177(4): 865-880. e21.（IF31.398）
2. Fan H, Hong B, Luo Y, et al. The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro[J]. Signal transduction and targeted therapy, 2020, 5(1): 1-3.(IF13.493)
3. Zhou L, Hou B, Wang D, et al. Engineering Polymeric Prodrug Nanoplatform for Vaccinatio Immunotherapy of Cancer[J]. Nano Letters, 2020.（IF12.279）
4. Wang J., et al., The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation[J]. Nat Commun. 2017 Aug 15;8(1):244.（IF 12.353）
5. Tao L, Yi Y, Chen Y, et al. RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages[J]. bioRxiv, 2020.( IF10.717)
6. Ma D, Zhao Y, Huang L, et al. A novel hydrogel-based treatment for complete transection spinal cord injury repair is driven by microglia/macrophages repopulation[J]. Biomaterials, 2020, 237: 119830.(IF10.317)
7. Lin Z, Xia S, Liang Y, et al. LXR activation potentiates sorafenib sensitivity in HCC by activating microRNA-378a transcription[J]. Theranostics, 2020, 10(19): 8834. (IF8.579) 11120ES
8. Liu D, Nie W, Li D, et al. 3D printed PCL/SrHA scaffold for enhanced bone regeneration[J]. Chemical Engineering Journal, 2019, 362: 269-279.（IF8.355）
9. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide PtrCLE20 inhibits vascular cambium activity in Populus[J]. Plant Biotechnology Journal, 2020, 18(1): 195-206.(IF8.154)
10. Chen L, Lam J C W, Tang L, et al. Probiotic modulation of lipid metabolism disorders caused by perfluorobutanesulfonate pollution in zebrafish[J]. Environmental Science & Technology, 2020.(IF7.864)
11. Zhao P, Zhang J, Wu A, et al. Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brain metastasis via macrophage-mediated innate immunity[J]. Journal of Controlled Release, 2020. ( IF7.727)
12. Xu X, Gao J, Dai W, et al. Gene activation by a CRISPR-assisted trans enhancer[J]. eLife, 2019, 8: e45973.（IF7.551）
13. Zhao Y, Wang H P, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions[J]. Bioresource Technology, 2020, 319: 124150.(IF7.539)
14. Li X, Zhang X, Zhao Y, et al. Cross-talk between gama-aminobutyric acid and calcium ion regulates lipid biosynthesis in Monoraphidium sp. QLY-1 in response to combined treatment of fulvic acid and salinity stress[J]. Bioresource Technology, 2020, 315: 123833.(IF7.539)
15. Nie, W., et al., Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering[J]. Carbon, 2017.116, 325-337.（IF 7.082）
16. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide Ptr CLE 20 inhibits vascular cambium activity in Populus[J]. Plant biotechnology journal, 2019.（IF6.84）
17. Peng L, Wang Y, Yang B, et al. Polychlorinated biphenyl quinone regulates MLKL phosphorylation that stimulates exosome biogenesis and secretion via a short negative feedback loop[J]. Environmental Pollution, 2020: 115606.(IF6.793)
18. Li A, Liu Q, Li Q, et al. Berberine reduces pyruvate-driven hepatic glucose production by limiting mitochondrial import of pyruvate through mitochondrial pyruvate carrier 1[J]. EBioMedicine, 2018, 34: 243-255. （IF6.68）
19. Lou M D, Li J, Cheng Y, et al. CREB mediates glucagon action to upregulate hepatic MPC1: inhibitory effect of ginsenoside Rb1 on hepatic gluconeogenesis[J]. British journal of pharmacology, 2019.（IF6.583）
20. Fan L, Ye H, Wan Y, et al. Adaptor protein APPL1 coordinates HDAC3 to modulate brown adipose tissue thermogenesis in mice[J]. Metabolism, 2019, 100: 153955.（IF6.513）
1. Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019, 177(4): 865-880. e21.（IF31.398）
2. Fan H, Hong B, Luo Y, et al. The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro[J]. Signal transduction and targeted therapy, 2020, 5(1): 1-3.(IF13.493)
3. Zhou L, Hou B, Wang D, et al. Engineering Polymeric Prodrug Nanoplatform for Vaccinatio Immunotherapy of Cancer[J]. Nano Letters, 2020.（IF12.279）
4. Wang J., et al., The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation[J]. Nat Commun. 2017 Aug 15;8(1):244.（IF 12.353）
5. Tao L, Yi Y, Chen Y, et al. RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages[J]. bioRxiv, 2020.( IF10.717)
6. Ma D, Zhao Y, Huang L, et al. A novel hydrogel-based treatment for complete transection spinal cord injury repair is driven by microglia/macrophages repopulation[J]. Biomaterials, 2020, 237: 119830.(IF10.317)
7. Lin Z, Xia S, Liang Y, et al. LXR activation potentiates sorafenib sensitivity in HCC by activating microRNA-378a transcription[J]. Theranostics, 2020, 10(19): 8834. (IF8.579) 11120ES
8. Liu D, Nie W, Li D, et al. 3D printed PCL/SrHA scaffold for enhanced bone regeneration[J]. Chemical Engineering Journal, 2019, 362: 269-279.（IF8.355）
9. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide PtrCLE20 inhibits vascular cambium activity in Populus[J]. Plant Biotechnology Journal, 2020, 18(1): 195-206.(IF8.154)
10. Chen L, Lam J C W, Tang L, et al. Probiotic modulation of lipid metabolism disorders caused by perfluorobutanesulfonate pollution in zebrafish[J]. Environmental Science & Technology, 2020.(IF7.864)
11. Zhao P, Zhang J, Wu A, et al. Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brain metastasis via macrophage-mediated innate immunity[J]. Journal of Controlled Release, 2020. ( IF7.727)
12. Xu X, Gao J, Dai W, et al. Gene activation by a CRISPR-assisted trans enhancer[J]. eLife, 2019, 8: e45973.（IF7.551）
13. Zhao Y, Wang H P, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions[J]. Bioresource Technology, 2020, 319: 124150.(IF7.539)
14. Li X, Zhang X, Zhao Y, et al. Cross-talk between gama-aminobutyric acid and calcium ion regulates lipid biosynthesis in Monoraphidium sp. QLY-1 in response to combined treatment of fulvic acid and salinity stress[J]. Bioresource Technology, 2020, 315: 123833.(IF7.539)
15. Nie, W., et al., Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering[J]. Carbon, 2017.116, 325-337.（IF 7.082）
16. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide Ptr CLE 20 inhibits vascular cambium activity in Populus[J]. Plant biotechnology journal, 2019.（IF6.84）
17. Peng L, Wang Y, Yang B, et al. Polychlorinated biphenyl quinone regulates MLKL phosphorylation that stimulates exosome biogenesis and secretion via a short negative feedback loop[J]. Environmental Pollution, 2020: 115606.(IF6.793)
18. Li A, Liu Q, Li Q, et al. Berberine reduces pyruvate-driven hepatic glucose production by limiting mitochondrial import of pyruvate through mitochondrial pyruvate carrier 1[J]. EBioMedicine, 2018, 34: 243-255. （IF6.68）
19. Lou M D, Li J, Cheng Y, et al. CREB mediates glucagon action to upregulate hepatic MPC1: inhibitory effect of ginsenoside Rb1 on hepatic gluconeogenesis[J]. British journal of pharmacology, 2019.（IF6.583）
20. Fan L, Ye H, Wan Y, et al. Adaptor protein APPL1 coordinates HDAC3 to modulate brown adipose tissue thermogenesis in mice[J]. Metabolism, 2019, 100: 153955.（IF6.513）
逆转录酶的前世今生：从莫洛尼鼠白血病病毒到定向进化改造
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