Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.
Q:细胞数要求 5×106,可以多于这个或者少于这个细胞数吗?
A:细胞数在 106-107是可以的,低于 5×106 细胞较少,提取的效果不好,多了的话,要多加裂解液,因为理解液过少会导致内源RNA酶裂解不充分,抑制不完全,导致RNA降解。不宜过多增加样本量,柱子有一定承载量,避免杂质去除不完全和RNA损失。
Q:柱子可以单独购买吗?
A:可以,货号为83666ES,包含柱子和收集管。
Published citations
[1] Yuan J, Wang JM, Li ZW, et al. Full-length transcriptome analysis reveals the mechanism of acupuncture at PC6 improves cardiac function in myocardial ischemia model. Chin Med. 2021;16(1):55. Published 2021 Jul 8. doi:10.1186/s13020-021-00465-8(IF:5.455)
[2] Lu Y , Shao M , Xiang H , Zheng P , Wu T , Ji G . Integrative transcriptomics and metabolomics explore the mechanism of kaempferol on improving nonalcoholic steatohepatitis. Food Funct. 2020;11(11):10058-10069. doi:10.1039/d0fo02123g(IF:4.171)
[3] Wu Q, He Y, Liu X, et al. Cancer stem cell-like cells-derived exosomal CDKN2B-AS1 stabilizes CDKN2B to promote the growth and metastasis of thyroid cancer via TGF-β1/Smad2/3 signaling [published online ahead of print, 2022 Jun 21]. Exp Cell Res. 2022;113268. doi:10.1016/j.yexcr.2022.113268(IF:3.905)
[4] Zhang J, Wang W, Zhu S, Chen Y. Increased SERPINA3 Level Is Associated with Ulcerative Colitis. Diagnostics (Basel). 2021;11(12):2371. Published 2021 Dec 16. doi:10.3390/diagnostics11122371(IF:3.706)
[5] Yang B, Wang F, Zheng G. Transmembrane protein TMEM119 facilitates the stemness of breast cancer cells by activating Wnt/β-catenin pathway. Bioengineered. 2021;12(1):4856-4867. doi:10.1080/21655979.2021.1960464(IF:3.269)
[6] Nie K, Cai M. SNAT2/SLC38A2 Confers the Stemness of Gastric Cancer Cells via Regulating Glutamine Level. Dig Dis Sci. 2022;67(7):2948-2956. doi:10.1007/s10620-021-07110-2(IF:3.199)
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