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Product data sheet PD-15210ES
Nt.BspQI (10 U/μL) Nt.BspQI (10 U/μL)
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Nt.BspQI (10 U/μL)

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分子克隆
Product no.15210ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15210ES84 现货 ¥1,218.00 促¥1,285.00
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Cat.No Spec Price Status
15210ES84 2000 U ¥1,218.00促¥1,285.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15210ES
Specification识别位置5'-GCTCTTCN↓-3'3'-CGAGAAGN-5'酶活10 U/μL反应条件1×Nt.BspQI Buffer;50℃孵育失活条件80℃温育20 min酶活定义单位活性单位(U)是指在 50 μL反应体系中,50℃ 1 h内
Origin翌圣生物科技(上海)股份有限公司
应用方法 分子克隆用于DNA分子的定点切口
生物特性参数 试剂类型切刻内切酶 应用在dsDNA底物上产生切口
样本类型 dsDNA
技术参数 浓度10 U/μL 缓冲液组分1×Nt.BspQI Buffer 储存条件-25~-15℃保存,有效期2年 识别位置5'-GCTCTTCN↓-3' / 3'-CGAGAAGN-5' 反应条件1×Nt.BspQI Buffer;50℃孵育 失活条件80℃温育20 min 酶活定义单位活性单位(U)是指在 50 μL反应体系中,50℃ 1 h内可以完全将1 µg的超螺旋pUC19 DNA转化成开环形式所需的酶量 甲基化修饰影响Dam、Dcm、CpG、EcoKI、EcoBI:无影响
实验类型 切刻内切酶在dsDNA底物上产生切口

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