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Product data sheet PD-15227ES
BpiI (10 U/μL) BpiI (10 U/μL)
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BpiI (10 U/μL)

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分子克隆Golden Gate组装酶切鉴定
Product no.15227ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15227ES72 现货 $158.00 促$165.00
Qty 1
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Cat.No Spec Price Status
15227ES72 250 U $158.00促$165.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15227ES
Specification识别位置5'-GAAGAC(N)2↓-3'3'-CTTCTG(N)6↑-5'反应条件1×FuniCut®缓冲液;37°C孵育失活条件80℃温育 20 min酶活定义1活性单位(U)是指在50 μL反应体系中,37℃ 1 h内完全酶切1 µg
Origin翌圣生物科技(上海)股份有限公司
应用方法 分子克隆用于常规克隆 Golden Gate组装用于Golden Gate组装 酶切鉴定用于DNA片段鉴定
生物特性参数 试剂类型限制性内切酶 应用Golden Gate组装、常规克隆或酶切鉴定
技术参数 浓度10 U/μL 储存条件-25~-15℃保存,有效期2年 识别位点5'-GAAGAC(N)2↓-3' 3'-CTTCTG(N)6↑-5' 反应条件1×FuniCut®缓冲液;37°C孵育 失活条件80℃温育 20 min 酶活定义1活性单位(U)是指在50 μL反应体系中,37℃ 1 h内完全酶切1 µg λDNA所需的酶量 同裂酶BstV2I BbsI 酶类型Type IIS型限制酶
实验类型 限制性酶切用于DNA片段切割和酶切鉴定 Golden Gate组装用于DNA无缝组装

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