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分子克隆

Product no.10922ES Sizes2 options Documents1 files SourceYeasen · Official

Cat. No. 10922ES20

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10922ES20 20 T ¥498.00促~~¥655.00~~ 现货 Enquire

10922ES50 50 T ¥948.00促~~¥1,355.00~~ 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No10922ES

Specification定向：可满足1-6个DNA片段定向插入简便：多片段连接一管内即可完成快速：最快5分钟即可完成连接反应高效：克隆生长数多，克隆阳性率可达95%以上

Origin翌圣生物科技(上海)股份有限公司

技术平台 分子克隆

应用方法 分子克隆基因克隆与DNA片段组装

生物特性参数 试剂类型同源重组克隆试剂盒 应用分子克隆

技术参数 反应温度50℃ 反应时间5-15 min 片段数量1-6个 片段长度50 bp-23 kb 载体量5 ng/20 μL反应体系 片段类型未纯化、纯化片段 克隆阳性率≥95% 储存条件-20℃保存，有效期1年 运输条件冰袋运输

实验类型 同源重组克隆将PCR产物定向克隆至载体，实现多片段顺序拼接

Documents & Citations

分子克隆产品手册 Open document

Published citations

[1] Tao R, Shi M, Zou Y, et al. Multicoloured fluorescent indicators for live-cell and in vivo imaging of inorganic mercury dynamics. Free Radic Biol Med. 2018;121:26-37. doi:10.1016/j.freeradbiomed.2018.04.562(IF:6.020)

[2] Guo Z, Long L, Ding S. Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Biosynthesis. Appl Biochem Biotechnol. 2020;192(3):935-951. doi:10.1007/s12010-020-03380-0(IF:2.277)