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Product data sheet PD-15060ES
FuniCut® XcmI FuniCut® XcmI
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FuniCut® XcmI

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分子克隆质粒线性化
Product no.15060ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15060ES56 现货 $158.00 促$165.00
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15060ES56 60 T $158.00促$165.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15060ES
Specification识别位置5'-CCA(N)5↓(N)4TGG-3'3'-GGT(N)4↑(N)5ACC-5'推荐反应条件1×FuniCut® 缓冲液;最适37℃孵育酶活5 U/μL失活条件80℃孵育20 min同裂酶无甲基化修饰影响Dam、Dcm、CpG、
Origin翌圣生物科技(上海)股份有限公司
应用方法 分子克隆载体构建 质粒线性化制备线性化质粒
生物特性参数 试剂类型限制性内切酶 应用分子克隆;载体构建;质粒线性化;快速切割质粒DNA、PCR产物、基因组DNA
技术参数 浓度5 U/μL 缓冲液组分1×FuniCut® 缓冲液 储存条件-25~-15℃保存,有效期2年 识别位置5'-CCA(N)5↓(N)4TGG-3' 3'-GGT(N)4↑(N)5ACC-5' 推荐反应条件1×FuniCut® 缓冲液;最适37℃孵育 失活条件80℃孵育20 min 同裂酶无 甲基化修饰影响Dam、Dcm、CpG、EcoKI、EcoBI:无影响
实验类型 限制性内切酶酶切用于DNA切割

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