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Product data sheet PD-15058ES
FuniCut® PspGI FuniCut® PspGI
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FuniCut® PspGI

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分子克隆载体构建质粒线性化
Product no.15058ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15058ES70 现货 ¥158.00 促¥165.00
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15058ES70 200 T ¥158.00促¥165.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15058ES
Specification识别位置5'...↑CCWGG...3'3'...GGWCC↑...5'推荐反应条件1×FuniCut® 缓冲液;最适37℃孵育酶活10 U/μL失活条件不可热失活,使用酚氯仿抽提或柱纯化同裂酶EcoRII, MvaI, BstNI, Bc
Origin翌圣生物科技(上海)股份有限公司
应用方法 分子克隆基因克隆 载体构建构建载体 质粒线性化线性化质粒
生物特性参数 试剂类型限制性内切酶 应用分子克隆,载体构建,质粒线性化
样本类型 质粒DNA PCR产物 基因组DNA
技术参数 酶活浓度10 U/μL 缓冲液组分1×FuniCut® 缓冲液 储存条件-25~-15℃保存,有效期2年 识别位点5'...↑CCWGG...3' 3'...GGWCC↑...5' 失活条件不可热失活,使用酚氯仿抽提或柱纯化 同裂酶EcoRII MvaI BstNI BciT130I BseBI AjnI Psp6I Bst2UI 甲基化修饰影响Dcm:序列完全重叠,剪切阻断 推荐反应条件1×FuniCut® 缓冲液;最适37℃孵育
实验类型 限制性内切酶酶切快速切割DNA

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